Sort   by:  
 Page size 
UnknownAnalysis of monoclonal immunoglobulins from bone marrow plasma cells using immunopurification and LC-MS.
Not available
S-EPMC10149385 | biostudies-literature
UnknownSimultaneous isotyping and semi-quantitation of anti-drug antibodies to an IgG1 biotherapeutic using hybrid LBA-LC-MS/MS.
Not available
S-EPMC11801339 | biostudies-literature
UnknownRapid immunopurification of mitochondria for metabolite profiling and absolute quantification of matrix metabolites.
Not available
S-EPMC5851482 | biostudies-literature
MetabolomicsChlorophyll biosynthesis under the control of arginine metabolism.

In natural environments, photosynthetic organisms adjust their metabolism to cope with the fluctuating availability of combined nitrogen sources, a growth-limiting factor. For acclimation, the dynamic degradation/synthesis of tetrapyrrolic pigments, as well as of the amino acid arginine, is ...

2024-03-12 | MTBLS8336 | MetaboLights
ProteomicsHuman LC-MS, TMEM216 inhibits breast cancer lung metastasis by modulating IGF1R-IRS4 signaling pathway
Immunopurification and mass spectrometry analysis of TMEM216-associated proteins. Cellular extracts from Flag-TMEM216-expressing 293FT cells were affinity-purified. The eluates were resolved by SDS-PAGE and stained with Coomassie Brilliant Blue. The protein bands were retrieved and analyzed by mass ...
ORGANISM(S): Homo sapiens (Human) 
2026-05-31 | PXD061739 | Pride
Human 293ft cells 
UnknownDevelopment and Validation of an LC-MS-Based Quantification Assay for New Therapeutic Antibodies: Application to a Novel Therapy against Herpes Simplex Virus.
Not available
S-EPMC7528202 | biostudies-literature
ProteomicsUrea artifacts interfere with immunopurification of lysine acetylation
Urea-based buffers can induce carbamylation artefacts. Here we report that carbamylation of lysine residues resembles the structure of acetylated lysines (K-ac) and compete during K-ac immunoaffinity purification. We propose an alternative strategy using an ionic detergent which improves significant...
ORGANISM(S): Homo sapiens (Human) 
2017-01-11 | PXD003701 | Pride
Urea Immunopurification Acetyl Acetylation Carbamylation Carbamyl 
ProteomicsLC-MS quantitative analysis of endosome-enriched lipidomic samples
The purpose of this experiment is to interrogate the lipid population present in purified endosomes to corroborate proteomic data in analyzing the effect of endosomal trafficking on membrane dynamics. This experiment will also validate whether endosomal-immunopurification (IP) is a viable approach f...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000088048 | MassIVE
ProteomicsSequential Peptide Immunopurification Methodology to Monitor the Crosstalk between SUMOylation and Ubiquitylation
We describe a method that permits the identification of proteins that are modified by both SUMOylation and ubiquitylation to better understand the role of this crosstalk. The procedure requires 3 days when starting from cell pellets and can yield more than 8000 SUMO sites and 3500 ubiquitin sites fr...
ORGANISM(S): Homo sapiens (Human) 
2018-03-27 | PXD006545 | Pride
Sumo Ubiquitin Shotgun proteomics 
ProteomicsIdentification of deubiquitinases controlled by extracellular matrix stiffness in cancer cells
The project was aimed at identifying deubiquitinases (DUBs) involved in cancer cell mechanosignaling. Lysates from cancer cells grown on collagen matrices with varying stiffnesses were incubated with an activity-based HA-tagged ubiquitin probe that covalently binds DUB active site. To identify DUBs ...
ORGANISM(S): Homo sapiens (Human) 
2025-08-22 | PXD054010 | Pride
Lc-ms/ms Immunopurification Dub abp Ecm stiffness 
Sort   by:  
 Page size