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ProteomicsFilamin C domain 18-21 in vitro kinase assay
In vitro kinase assay with human filamin C domain 18-21 recombinantly expressed in E.coli, purified by Ni2+-NTA beads and incubated with PKC alpha, Akt or a combination of both to identify kinase specific phosphorylation sites.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-25 | PXD008893 | Pride
Filamin c Akt In vitro kinase assay Pkb Pkcalpha 
ProteomicsHigh-throughput identification of FLT3 wild-type and mutant kinase substrate preferences and application to design of sensitive in vitro kinase assay substrates
Abnormalities in the FLT3 signaling pathway play an integral role in AML disease relapse and drug resistance. Developing new and specific FLT3 tyrosine kinase inhibitors for use in combination to induction therapy is an important step to reduce disease relapse and achieve clinical remission. To deve...
ORGANISM(S): Homo sapiens (Human) 
2019-01-07 | PXD011478 | Pride
Kinase assay linked with phosphoproteomics Flt3 Acute myeloid leukemia Mass spectrometry Phosphoproteomics 
ProteomicsIn vitro kinase assay Pim1/GBP1
High resolution mass spectrometry-based analysis of an in vitro kinase assay to dissect the PIM1 kinase/GBP1 substrate relationship.
ORGANISM(S): Homo sapiens (Human) 
2023-09-26 | PXD030010 | Pride
Phospho Kinase assay Human 
ProteomicsLarge-scale in vitro kinase assay with FLNc d23-24 and PKC alpha
Large-scale in vitro kinase assay with human and murine FLNc and its site mutants and PKC alpha.
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2017-01-02 | PXD003185 | Pride
Lc-ms In vitro kinase assay Flnc Pkc alpha 
ProteomicsIdentifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry
Mass spectrometry‐based in vitro kinase screens play an essential role in the discovery of kinase substrates, however, many suffer from biological and technical noise or necessitate genetically‐altered enzymecofactor systems. We describe a method that combines stable γ‐[18O2]‐ATP with classical in v...
ORGANISM(S): Homo sapiens (Human) 
2018-10-17 | PXD002133 | Pride
Human Kinase substrates  in vitro kinase assay  proteomics  mass spectrometry  heavy atp  non receptor tyrosine kinase abl1 
ProteomicsCyclin dependent kinase 5 (CDK5) regulates the circadian clock
We show that the cyclin-dependent kinase 5 (CDK5) regulates the mammalian circadian clock via phosphorylation of PER2. CDK5 phosphorylated PER2 at serine residue 394 (S394) as shown by an in vitro kinase assay.
ORGANISM(S): Mus musculus (Mouse) 
2019-12-04 | PXD012068 | Pride
Mouse In vitro kinase assay Phosphorylation Per2 
ProteomicsNaringenin targets PI3K p85alpha to suppress PI3K/AKT signaling pathway and ameliorate disordered MMP-9 secretion in cigarette smoke extract-induced alveolar macrophages in vitro
Naringenin has demonstrated potential therapeutic effects against cigarette smoke-induced lung injury; however, its underlying mechanisms of regulating matrix metalloproteinase-9 (MMP-9) in alveolar macrophages remain unclear. Methods: The regulatory mechanisms of naringenin in cigarette smoke extra...
ORGANISM(S): Mus Musculus 
PXD063137 |
ProteomicsIdentification of phosphorylation sites of purified ORP8 from in vitro AMPK kinase assay
We investigated the potential role of AMPK in the phosphorylation of ORP8.In vitro kinase assay using purified ORP8 and kinase-active AMPK complex confirmed that ORP8 is the direct substrate of AMPK. Phosphorylated ORP8 from in vitro kinase assay was analyzed by HPLC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD036201 | Pride
Ampk Phosphorylation Orp8 
ProteomicsIdentification of ABL Kinase Substrates by Kinase Assay Linked Phosphoproteomics and Multiple Drug Treatments
The kinase assay linked phosphoproteomics (KALIP) strategy was used to identify direct substrates of the Abelson tyrosine kinase (ABL). First, kinase assays using peptides and proteins from chronic myelogenous leukemia cells and recombinant ABL were done to identify kinase substrates in vitro. The A...
ORGANISM(S): Homo Sapiens (human) 
2019-03-20 | PXD005431 | JPOST Repository
ProteomicsFenbendazole Met ID_in vitro_cross-species_FBMN
Fenbendazole Met ID by an integrative approach using feature-based molecular networking of cross-species in vitro liver microsomal and hepatocellular incubation.
ORGANISM(S): Canis Lupus Familiaris (ncbitaxon:9615) Macaca Fascicularis (ncbitaxon:9541) Cd1 Mouse Homo Sapiens (ncbitaxon:9606) 
2024-08-08 | MSV000095554 | MassIVE
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