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ProteomicsProteomic Sample Preparation in the Low Microgram Range – A Critical Evaluation of Current Workflows
Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive quantitative bottom-up proteomics workflow. Over the past years, several protocols been specifically developed for the preparation of samples, which are limited in quantity. In the present study, we carried ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2018-05-11 | PXD006760 | Pride
Single-pot solid-phase-enhanced sample preparation (sp3) Filter-aided sample preparation (fasp) In-stagetip digestion (ist) Sample preparation Bottom-up proteomics 
ProteomicsComparative evaluation of different sample preparation workflows for reproducible, quantitative, and in-depth analysis of urine proteomics
The growing field of urinary proteomics has provided a promising opportunity to identify biomarkers useful for diagnosis and prognostication of a number of diseases. Urine is abundant and readily collected in a non-invasive manner which makes it eminently available for testing, however sample prepar...
ORGANISM(S): Homo sapiens (Human) 
2020-03-06 | PXD016273 | Pride
Reproducibility Urine proteomics Mass spectrometry Preomics ist Mstren Sample preparation Label-free S-trap 
ProteomicsProteomic Analysis of Urinary Extracellular Vesicles Reveals a Role for the Complement System in Medullary Sponge Kidney Disease
Medullary sponge kidney (MSK) disease is a rare and neglected kidney condition often associated with nephrocalcinosis/nephrolithiasis and cystic anomalies in the precalyceal ducts. Little is known about the pathogenesis of this disease, so we addressed the knowledge gap using a proteomics approach. ...
ORGANISM(S): Homo sapiens (Human) 
2021-12-09 | PXD025547 | Pride
Medullary sponge kidney  idiopathic calcium nephrolithiasis  complement system  proteomics 
ProteomicsProteomic Profiling of Retinoblastoma-Derived Exosomes reveals potential biomarkers of vitreous seeding
Retinoblastoma (RB) is the most common tumor of the eye in early childhood. Despite recent advances in conservative treatment have greatly improved the visual outcome, local tumor control remain difficult in presence of massive vitreous seeding. Traditional biopsy has long been considered unsafe in ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD016488 | Pride
Exosomes Proteomics Retinoblastoma Biomarkers 
ProteomicsFrontiers in Plasma Proteome Profiling Platforms: Innovations and Applications.
Biomarkers play a crucial role in advancing precision medicine by enabling more targeted and individualized approaches to diagnosis and treatment. Various biofluids, including serum, plasma, cerebrospinal fluid (CSF), saliva, tears, pancreatic cyst fluids, and urine, have been identified as rich sou...
ORGANISM(S): Homo sapiens (Human) 
2024-07-03 | PXD050425 | Pride
Biomarker Sp100 instrument Biofluids proteome profiling Preomics-ist Seer proteograph Timstofpro2 Secretome Plasma proteome 
ProteomicsProteomics of aqueous humor for biomarkers discovery in retinoblastoma
Background: Recent therapeutic advances have greatly improved the eye preservation rate in patients affected by retinoblastoma (RB), however local tumor control remains more difficult in the presence of resistant and/or relapsing retinal or vitreous disease. Thus, the identification of new biomarker...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD022218 | Pride
Proteomic analysis Retinoblastoma Humor aqueous Biomarkers 
ProteomicsProteomics of aqueous humor for biomarkers discovery in retinoblastoma (pt2)
Background: Recent therapeutic advances have greatly improved the eye preservation rate in patients affected by retinoblastoma (RB), however local tumor control remains more difficult in the presence of resistant and/or relapsing retinal or vitreous disease. Thus, the identification of new biomarker...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD022256 | Pride
Proteomic analysis Retinoblastoma Humor aqueous Biomarkers 
ProteomicsPhosphoproteomics reveals the GSK3-PDX1 axis as a key pathogenic signaling node in diabetic islets
Progressive decline of pancreatic beta cell function is central to the pathogenesis of type 2 diabetes. Protein phosphorylation regulates glucose-stimulated insulin secretion from beta cells, but how signaling networks are remodeled in diabetic islets in vivo remains unknown. Using high-sensitivity ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-03-15 | PXD012671 | Pride
Gsk3 Islets Proteomics Mass spectrometry Pdx1 Signalling networks Insulin secretion Diabetes Phosphoproteomics 
ProteomicsDeep palaeoproteomic profiling of archaeological human brains
Palaeoproteomics leverages the persistence, diversity, and biological import of ancient proteins to explore the past, and answer fundamental questions about phylogeny, environment, diet, and disease. These insights are largely gleaned from hard tissues like bone and teeth, as well-established protoc...
ORGANISM(S): Homo sapiens (Human) 
2025-04-02 | PXD062254 | Pride
Brain  palaeoproteomics  lc-ms/ms  ion mobility  faims 
ProteomicsProteomic analysis of ARPE-19 cells exposed to chronic H2O2 treatment
We performed a proteomic analysis of a retinal pigment epithelium cell line (ARPE-19) exposed to long-term sublethal levels of H2O2 to mimic low-level pro-oxidative insult in age-related macular degeneration (AMD). using a wide range of biochemical analyses and cell culture techniques, we highlighte...
ORGANISM(S): Homo sapiens (Human) 
2021-01-04 | PXD022545 | Pride
Arpe-19 Retinal pigment epithelium Oxidative stress Macular degeneration 
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