Sort   by:  
 Page size 
TranscriptomicsHuman cortical organoids. Chronic treatment with GSK3 inhibitor CHIR99021. Bulk RNAseq.
Here we used human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Cortical organoids were dif...
ORGANISM(S): Homo sapiens 
2020-06-04 | E-MTAB-8325 | biostudies-arrayexpress
TranscriptomicsTransformants of wheat GSK3/Shaggy-like kinase TaSK5 vs wild-type Col-0
A novel cold-inducible GSK3/Shaggy-like kinase cDNA (TaSK5) was isolated from winter wheat by a macroarray-based differential screening approach. Sequence analysis of TaSK5 revealed high similarity to Arabidopsis subgroup I GSK3/Shaggy-like kinases ASK-alpha, ASK-gamma and ASK-epsilon. Transgenic Ar...
ORGANISM(S): Arabidopsis thaliana 
2014-11-02 | E-GEOD-59556 | biostudies-arrayexpress
TranscriptomicsPerturbation of the Akt/Gsk3-beta signaling pathway is common to Drosophila expressing expanded untranslated CAG, CUG and AUUCU repeat RNAs
Recent evidence supports a role for RNA as a common pathogenic agent in both the “polyglutamine” and “untranslated” dominant expanded repeat disorders. One feature of all repeat sequences currently associated with disease is their predicted ability to form a hairpin secondary structure at the RNA...
ORGANISM(S): Drosophila melanogaster 
2011-04-28 | E-GEOD-27178 | biostudies-arrayexpress
ProteomicsGSK3 phosphorylation sites in Lpd
Neural crest cells are multipotent cells that delaminate from the neuroepithelium, migrating throughout the embryo. Aberrant migration causes developmental defects. Animal models are improving our understanding of neural crest anomalies, but in vivo migration behaviours are poorly understood. Here, ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-05 | PXD044150 | Pride
Gsk3 Phosphorylation Cid/etd tandem mass spectrometry Lpd 
ProteomicsRegulatory phosphorylation of eIF6 by GSK3
The overall goal of this project is to determine the key sites in the C-terminus of eIF6 that are recognized and phosphorylated by GSK3. Analysis of kinase reactions using the site-specific mutants as well as the C-terminus deletion mutants of eIF6 indicated that there are GSK3-specific motifs in th...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-06-16 | MSV000085596 | MassIVE
ProteomicsPolyubiquitination analysis upon GSK3 inhibition in HeLa cell extracts
HeLa cell extracts with or without GSK3 enzyme inhibition were assayed using protein microarrays in order to detect GSK3-dependent changes in protein polyubiquitination. HeLa lysates in triplicates were supplemented with ubiquitin and incubated on protein microarrays (ProtoArray 5.0; Invitrogen) in ...
ORGANISM(S): Homo sapiens 
2014-05-15 | E-GEOD-50629 | biostudies-arrayexpress
TranscriptomicsSingle cell RNAseq of human cortical organoids investigating the effect of chronic treatment with GSK3 inhibitor CHIR99021
Here we used human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Cortical organoids were dif...
ORGANISM(S): Homo sapiens 
2020-06-09 | E-MTAB-8337 | biostudies-arrayexpress
ProteomicsRegulation of Glycogen Synthase kinase 3beta by phosphorylation and O-beta-linked N-acetylglucosaminylation: implications on tau protein phosphorylation
Glycogen synthase kinase 3 (GSK3) plays a pivotal role in signaling pathways related to insulin metabolism and in the pathogenesis of Alzheimer s disease (AD) and other neurodegenerative disorders. More particularly, the GSK3beta isoform is involved in AD-associated tau pathology as one of the major...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-02-09 | MSV000094049 | MassIVE
ProteomicsApplication of TurboID-mediated proximity labeling and phosphoproteomics for mapping a GSK3 kinase signaling network in Arabidopsis
Transient protein-protein interactions (PPIs), such as those between posttranslational modifying enzymes and their substrates, play key roles in cellular regulation, but are difficult to identify. Here we demonstrate the application of enzyme-catalyzed proximity labeling (PL), using the engineered p...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-05-10 | PXD017085 | Pride
Proximity labeling Turboid Mass spectrometry Protein interactome Protein-protein interaction Gsk3 kinase Phosphoproteomics Plant Biotinylation 
ProteomicsPhosphoproteomics reveals the GSK3-PDX1 axis as a key pathogenic signaling node in diabetic islets
Progressive decline of pancreatic beta cell function is central to the pathogenesis of type 2 diabetes. Protein phosphorylation regulates glucose-stimulated insulin secretion from beta cells, but how signaling networks are remodeled in diabetic islets in vivo remains unknown. Using high-sensitivity ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-03-15 | PXD012671 | Pride
Gsk3 Islets Proteomics Mass spectrometry Pdx1 Signalling networks Insulin secretion Diabetes Phosphoproteomics 
Sort   by:  
 Page size