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MetabolomicsIAPP toxicity activates HIF1α/PFKFB3 signaling delaying β-cell loss at the expense of β-cell function
The islet in type 2 diabetes (T2D) is characterized by amyloid deposits derived from islet amyloid polypeptide (IAPP), a protein co-expressed with insulin by β-cells. In common with amyloidogenic proteins implicated in neurodegeneration, human IAPP (hIAPP) forms membrane permeant toxic oligomers imp...
2020-04-17 | MTBLS951 | MetaboLights
TranscriptomicsTranscription profiling of human islet cells CD40L vs islet cells controls
Expression of CD40 in non-hematopoietic cells has been linked to inflammation. We presented evidence that CD40, a T-cell costimulatory molecule, is expressed in human β-cells and the engagement of CD40 in insulinoma cells activated the NFKB and ERK1/2 pathways. CD40 activation in human islets cell...
ORGANISM(S): Homo sapiens 
2008-10-20 | E-GEOD-8873 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse MIN6 insulinoma beta cells following knock-down of murine Pdx1
In order to identify genes that are regulated by Pdx1, MIN6 insulinoma cells were transduced with adenovirus expressing a short hairpin RNA directed against murine Pdx1 (AdshPdx1) or luciferase (AdshLuc) or not transduced at all (untreated). RNA was harvested and reverse transcribed. Reduced expre...
ORGANISM(S): Mus musculus 
2010-01-01 | E-MTAB-132 | biostudies-arrayexpress
TranscriptomicsChIP-Chip and SACO Analysis of Pdx-1 binding in NIT-1 Insulinoma Cells
The BCBC Promoter Chip 5B was used to identify genomic targets of Pdx-1 binding. Genomic DNA from mouse NIT-1 insulinoma cells was immunoprecipitated with a Pdx-1 Antibody, PCR amplified, and labeled to the chip. All hybridizations were versus a common reference sample which was a labeled IgG IP. ...
ORGANISM(S): Mus musculus 
2014-11-21 | E-MTAB-3126 | biostudies-arrayexpress
ProteomicsProteoglycan profiling of human, rat and mouse insulin-secreting cells
Proteoglycans (PGs) are proteins with glycosaminoglycan (GAG) chains, such as chondroitin sulfate (CS) or heparan sulfate (HS), attached to serine residues. We have earlier shown that prohormones can carry CS, thus, constituting a novel class of PGs. The mapping of GAG modifications of proteins in e...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Rattus norvegicus (Rat) 
2022-02-16 | PXD024230 | Pride
Glycosaminoglycan Insulinoma islet cells Chromogranin-a Insulin Proteoglycan 
TranscriptomicsExpression data from mouse insulinoma cells
We previously isolated a subclone, MIN6 clone 4, from the parental MIN6 cells, that shows well-regulated insulin secretion in response to glucose, glybenclamide, and KCl, even after prolonged culture. To investigate the molecular mechanisms responsible for preserving GSIS in this subclone, we compar...
ORGANISM(S): Mus musculus 
2013-04-22 | E-GEOD-43774 | biostudies-arrayexpress
GenomicsAcute IL-1beta exposure in rat insulinoma cells and human islets
Acute IL-1beta exposure in rat insulinoma cells and human islets
PRJNA510929 | ENA
TranscriptomicsFeedback Inhibition of CREB Signaling Promotes Beta Cell Dysfunction in Insulin Resistance.
Although persistent elevations in circulating glucose concentrations promote compensatory increases in pancreatic islet mass, unremitting insulin resistance causes a deterioration in beta cell function that disrupts glucose balance and signals the progression to diabetes 1. Glucagon like Peptide 1 (...
ORGANISM(S): Rattus norvegicus 
2015-02-23 | E-GEOD-60158 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse pancreatic beta cell line (MIN6) treated with amylin at different times and doses and growth at different concentrations of glucose
Murine pancreatic beta cell line MIN6 was growth at two different concentrations of glucose (22,2 and 5,5 mM of glucose), 37ºC, 5% CO2 and was treated at four different concentrations of human amylin (0, 1, 10 and 20 uM) during three different times (2, 12 and 24 hours)
ORGANISM(S): Mus musculus 
2009-04-10 | E-GEOD-2253 | biostudies-arrayexpress
TranscriptomicsC4b-binding protein protects beta-cells from islet amyloid polypeptide induced cytotoxicity.
Complement inhibitor C4b-binding protein (C4BP) is synthesized in liver and pancreas and composed of 7 identical alpha chains and one unique beta chain. We showed previously that C4BP binds islet amyloid polypeptide (IAPP) and affects fibril formation in vitro. Now we found that polymeric C4BP inhib...
ORGANISM(S): Rattus norvegicus 
2016-08-25 | E-GEOD-79224 | biostudies-arrayexpress
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