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ProteomicsHuman serum N-linked intact glycopeptides LC-MS/MS
Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) in human serum is challenging due to the wide dynamic range of serum protein abundances, the lack of a complete serum N-Glycan database and the existence of non-specifical...
ORGANISM(S): Armoracia rusticana Homo sapiens (Human) Gallus gallus (Chicken) 
2020-04-21 | PXD015622 | Pride
N-linked intact glycopeptide Co-elution Spectral library search Human serum Isotopic distribution 
ProteomicsHydrophilic interaction liquid chromatography contributes to integrated N-glycoproteomics analysis of human saliva for lung cancer
We aimed to in-depth characterize and quantify salivary N-glycoproteomics. HILIC enrichment and LC-MS/MS were combined to investigate salivary glycosylation both at deglycopeptides level and glycopepeptides level, and alterations in site-specific glycoforms for human saliva were detected between lun...
ORGANISM(S): Homo sapiens (Human) 
2023-02-08 | PXD030670 | Pride
Hilic Lung cancer N-glycosites Saliva Intact n-glycopeptides 
ProteomicsOptimal Dissociation Methods Differ for N- and O-glycopeptides
Site-specific characterization of glycosylation requires intact glycopeptide analysis, and recent efforts have focused on how to best interrogate glycopeptides using tandem mass spectrometry (MS/MS). Beam-type collisional activation, i.e., higher-energy collisional dissociation (HCD), has been a val...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-07-20 | PXD017646 | Pride
Glycoproteomics N-glycopeptides Scehcd Ethcd O-glycopeptides Etd 
ProteomicsIntact N-glycopeptides and proteomics from HCC tissue tumors with low and high AFP concentrations
Hepatocellular carcinoma (HCC) is still one of the malignant tumors with high morbidity and mortality in China and worldwide. Although AFP have been widely used as important biomarkers for HCC diagnosis and evaluation, the AFP level has a huge variation among HCC patient populations. Understanding t...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD016406 | Pride
Proteomics Intact glycopeptide Mass spectrometry Hepatocellular carcinoma 
ProteomicsDirect glycan structure determination of intact N-linked glycopeptides by low-energy collision-induced association tandem mass spectrometry and predicted spectral library searching
Intact glycopeptide MS analysis to reveal site-specific protein glycosylation is an important frontier of proteomics. However, computational tools for analyzing MS/MS spectra of intact glycopeptides are still limited and not well-integrated into existing workflows. In this work, a novel computationa...
ORGANISM(S): Homo sapiens (Human) 
2016-08-17 | PXD002803 | Pride
Glycopeptide Lc-msms 
ProteomicsCharacterization of sialylated N- and O-glycopeptides from complex proteomes on an Orbitrap Fusion Tribrid mass spectrometer by Isotope Targeted Glycoproteomics (IsoTaG). Application to azido and alkynyl sugars
Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation. Correlation of the intact glycoform to the protein attachment site is a critical step to assign functional roles to specific glycoproteins. Isotope targeted...
ORGANISM(S): Homo sapiens (Human) 
2017-02-22 | PXD004302 | Pride
Lc-ms/ms Tissue culture Glycoproteomics Metabolic labeling 
ProteomicsImproving quantitative glycoproteomics by systematic evaluation of different glycopeptide enrichment, quantification, and mass spectrometry strategies
In this study, we conducted a systematic investigation into a glycoproteomic workflow. Firstly, Label-free and TMT labeling strategies were utilized for intact N-glycopeptide quantification. A total of 2,924 unique intact N-glycopeptides can be quantified after being labeled using TMT approach. Comp...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2025-05-07 | PXD054874 | Pride
Glycoproteomics Intact glycopeptide Identification Quantification 
ProteomicsAutoGP 2: an improved fully automated high-throughput system for simultaneously enriching and separating intact glycopeptides
An improved fully automated high-throughput system, named as AutoGP 2, for simultaneously enriching and separating intact glycopeptides has been developed in this study by introducing a second 10-port valve (valve 2) to the previously developed AutoGP system with only a 10-port valve (valve 1). Spec...
ORGANISM(S): Mus Musculus 
PXD069435 |
ProteomicsLC-MS/MS analysis of maize seedling leaf bases
1 g samples of maize seedling leaf bases were collected from the wild-type (Zheng58) plants and the mutants (bzu3-2). we undertook glycoproteomics analyses of the N-glycans. Based on the libraries of maize protein group and plant N-glycosylation modification in the Uniprot database, we identified 2,...
ORGANISM(S): Zea mays (Maize) 
2023-07-24 | PXD041642 | Pride
N-glycopeptides Maize Lc-ms/ms 
ProteomicsSite-specific N-glycan Analysis of Neonatal Mouse Hearts
The heart of a newborn mouse has an exceptional capacity to regenerate from myocardial injury but lose it after a week of life, which has been utilized as a valuable model to explore the cues for heart regeneration. More and more researches indicated that glycoprotein played an important role in car...
ORGANISM(S): Mus musculus (Mouse) 
2020-05-26 | PXD017139 | Pride
Neonatal mouse heart Intact glycopeptides Site-specific glycosylation Glycoproteomics Lc-ms/ms 
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