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ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - 80 compound screen (HEK293T and Jurkat)
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiob...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054127 | Pride
Chemoproteomics Data independant acquisition Label-free qauntification 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - Library Build, concentration response, structure activity relationships and in-cell treatment
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiob...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054145 | Pride
Chemoproteomics. lavel-free quantification Data independant acquisition 
ProteomicsProteome-Wide Target Identification Using Reactive Metallo-Scaffolds: A Platform for Metallodrug Discovery
Metal complexes offer unique opportunities as scaffolds in chemical biology and drug discovery, with tuneable geometry, modular coordination environments, and structural features not readily accessible with organic molecules. Here, we introduce reactive metallo-scaffolds (r-mS) - a new class of cova...
ORGANISM(S): Homo sapiens (Human) 
2026-09-21 | PXD068320 | Pride
Reactive metalloscaffolds Dia-pasef Ia-dtb 
ProteomicsEnantioselective OTUD7B fragment discovery through chemoproteomics screening and high-throughput optimisation
Deubiquitinating enzymes (DUBs) are key regulators of cellular homeostasis, and their dysregulation is associated with several human diseases. The ovarian tumour protease (OTU) family of DUBs are biochemically well-characterised and of therapeutic interest, yet only a few tool compounds exist to stu...
ORGANISM(S): Homo sapiens (Human) 
2025-01-13 | PXD057851 | Pride
Dubs Dia Iodoacetamide-desthiobiotin (ia-dtb) Chemoproteomics Cysteine reactivity profiling Abpp 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - HEK293T - Global expression proteomics
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. This platform profiles cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiobiotin (IA-...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054105 | Pride
Data independant acquistion Label free quantification 
ProteomicsCovalent fragment screening to inhibit the E3 ligase activity of bacterial NEL enzymes SspH1 and SspH2
As the global fight against antimicrobial resistance in bacteria becomes increasingly pressing, new tool compounds are needed to understand and identify novel therapeutic targets. Here, cysteine-directed fragment-based drug discovery is coupled with a high throughput chemistry direct-to-biology scre...
ORGANISM(S): Homo sapiens (Human) 
2025-11-10 | PXD057304 | Pride
Bacterial nels Fragment-based drug discovery Covalent fragments Salmonella effectors Chemoproteomics E3 ligase High throughput chemistry 
ProteomicsCysteine reactivity profiling identifies host regulators of Mycobacterium tuberculosis replication in human macrophages
Innate immune cells such as monocytes and macrophages provide the earliest defense against infection by intracellular pathogens by initiating signaling pathways and restricting pathogen replication. However, the full complement of proteins that mediate cell-autonomous immunity remains incompletely d...
ORGANISM(S): Homo sapiens (Human) 
2026-03-02 | PXD067709 | Pride
Innate immunity Cysteine Macrophage Mycobacterium tuberculosis Mass spectrometry Chemical proteomics Covalent probes 
ProteomicsSystematic identification of anticancer drug targets reveals a nucleus-to-mitochondria ROS sensing pathway
Multiple anticancer drugs have been proposed to cause cell death, in part, by increasing the steady-state levels of cellular reactive oxygen species (ROS). However, for most of these drugs exactly how the resultant ROS function and are sensed is poorly understood. It remains unclear which proteins t...
ORGANISM(S): Homo sapiens (Human) 
2024-06-16 | PXD041138 | Pride
Chermoresistance  redox signaling Ros  chemical proteomics  organelle signaling  chemotherapy 
ProteomicsProteomic Cysteine Profiling of DMI- and DMF-treated MEFs
Dimethyl fumarate (DMF) and dimethyl itaconate (DMI) are electrophilic metabolite derivatives with potent immunomodulatory properties. While DMF is clinically approved for multiple sclerosis, the molecular mechanisms underlying the actions of both compounds remain incompletely understood. Both have ...
ORGANISM(S): Mus musculus (Mouse) 
2026-09-18 | PXD080692 | Pride
Mouse Cysteine reactivity profiling Mef Abpp 
ProteomicsGenetic and pharmacological inactivation of peptidoglycan remodeling increases antibiotic susceptibility of vancomycin-resistant Enterococcus faecium
Vancomycin-resistant Enterococcus faecium (VREfm) is a leading cause of healthcare-associated infections globally and demands new approaches for treatment. Here we show that genetic and pharmacological inactivation of a highly conserved NlpC/P60 peptidoglycan hydrolase, secreted antigen A (SagA), en...
ORGANISM(S): Enterococcus faecium ERV165 
2026-04-13 | PXD075040 | Pride
Antibiotic adjuvant Covalent inhibitor Chemoproteomics Vancomycin-resistant enterococcus faecium Peptidoglycan hydrolase 
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