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ProteomicsQuantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology
A new analytical method capable of simultaneously assessing cellular protein expression and cysteine oxidation would provide a valuable tool for the field of cysteine-targeted biology. Here, we show a workflow based on protein quantification using metabolic labeling and determination of cysteine oxi...
ORGANISM(S): Homo sapiens (Human) 
2026-09-03 | PXD012504 | Pride
Cysteine Liquid chromatography/mass spectrometry Silac-iodotmt labeling Peroxiredoxin Proteome Redoxome 
ProteomicsRedox-proteomic analysis of pancreatic β-cells upon glucose stimulation
Redox signaling mediated by reversible oxidative cysteine thiol modifications is crucial for driving cellular adaptation to dynamic environmental changes, maintaining homeostasis, and ensuring proper function. This is particularly critical in pancreatic β-cells, which are highly metabolically active...
ORGANISM(S): Mus musculus (Mouse) Rattus norvegicus (Rat) 
2024-09-30 | PXD052765 | Pride
Iodotmt Redox proteomics 
ProteomicsPeroxiredoxin 6 protects irradiated cells from oxidative stress and shapes their senescence-associated cytokine landscape, redox proteomic analysis
Cellular senescence is a complex stress response defined as an essentially irreversible cell cycle arrest mediated by the inhibition of cell cycle-specific cyclin dependent kinases. The imbalance in redox homeostasis and oxidative stress have been repeatedly observed as one of the hallmarks of the s...
ORGANISM(S): Homo sapiens (Human) 
2022-02-16 | PXD028780 | Pride
Peroxiredoxin 6 Cellular senescence Silac-iodotmt Ionizing radiation Redox proteomics 
ProteomicsRedox proteomics analysis of Arabidopsis wild type and grxs12 mutants
For an overall evaluation of the protein redox status in grxs12 leaves, the iodoTMT-based redox proteomics approach was employed. After protein extraction, the free thiols in Cys were NEM-blocked, and then the Cys thiols with reversible oxidations such as sulfenic acids (-SOH), S-glutathionylation (...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-01-16 | PXD059068 | Pride
Arabidopsis Redox proteomics Grxs12 mutants Iodotmt label 
ProteomicsIdentification of heat induced proteins in young tomato flower buds
Tomato flowering and fruit set require an optimal temperature of 25/22 ± 2˚C (day/night). When the air temperature reaches to above the optimal range (higher than 30/26˚C; day/night), only a small number of flower buds would develop into mature flowers and produce a reduced number of pollen. This p...
ORGANISM(S): Solanum lycopersicum x Solanum tuberosum 
2022-03-03 | PXD012160 | Pride
Iodotmt  tomato flower 
ProteomicsArabidopsis high light redox proteomics
DDA experiment investigating differential cystein redox modification using iodoTMT label on high light treated samples from Arabidsopsis thaliana leaves.
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2024-08-23 | PXD048299 | JPOST Repository
ProteomicsS-glutathionylation proteome profiling of Streptococcus mutans
S-glutathionylation is an important post-translational modification (PTM) process that targets the protein cysteine thiol by the addition of glutathione (GSH). This modification can prevent proteolysis from over-oxidation of protein cysteine residue during the condition of oxidative or nitrosative s...
ORGANISM(S): Streptococcus mutans UA159 
2020-07-14 | PXD019564 | Pride
S-glutathionylation  streptococcus mutans  post-translational modification 
ProteomicsOrphan precursor proteins and ROS activate the mitochondrial UPR_Redox proteomics
The mitochondrial unfolded protein response (UPRmt) safeguards mitochondria from proteotoxic damage by activating a dedicated transcriptional response in the nucleus to restore proteostasis. How the mitochondrial protein misfolding information is signalled to the nucleus as part of the human UPRmt r...
ORGANISM(S): Homo sapiens (Human) 
2023-05-19 | PXD032011 | Pride
Uprmt Proteomics Iodotmt Ros Mitochondria Redox 
ProteomicsCharacterization of the redox proteome of Drosophila melanogaster S2 cells and w1118 fly eyes.
Reactive oxygen species (ROS) serve as intracellular signaling molecules; however, in excess ROS molecules are damaging to biomacromolecules such as DNA, lipids, and proteins. The excess accumulation of ROS leads to oxidative stress, disrupting redox homeostasis in the cell and has been linked with ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-04-27 | PXD034050 | Pride
Drosophila melanogaster schneider s2 cells Drosophila eye Redox proteome Blue light 
ProteomicsComprehensive redox profiling of the thiol proteome of Clostridium difficile
The strictly anaerobic bacterium C. difficile has become one of the most problematic hospital acquired pathogens and a major burden for health care systems. Although antibiotics work effectively in most C. difficile infections (CDIs), their detrimental effect on the intestinal microbiome paves the w...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2018-03-05 | PXD007278 | Pride
Thiol redox proteomics Clostridium difficile 
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