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ProteomicsComparative proteomics using iodoTMT to identify changes in protein expression and cysteine oxidation in Leishmania mexicana
Two cell-lines of Leishmania mexicana were investigated using a TMT-based approach; wild-type and an attenuated H-line. Half of each sample was fully reduced and all cysteine residues were labelled with the cysteine-reactive iodoTMT reagent, while the other half was reacted with N-ethylmaleimide to ...
ORGANISM(S): Leishmania mexicana MHOM/GT/2001/U1103 
2018-11-23 | PXD010938 | Pride
Leishmania mexicana Tmt Cysteine oxidation Comparative proteomics 
ProteomicsQuantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology
A new analytical method capable of simultaneously assessing cellular protein expression and cysteine oxidation would provide a valuable tool for the field of cysteine-targeted biology. Here, we show a workflow based on protein quantification using metabolic labeling and determination of cysteine oxi...
ORGANISM(S): Homo sapiens (Human) 
2026-09-03 | PXD012504 | Pride
Cysteine Liquid chromatography/mass spectrometry Silac-iodotmt labeling Peroxiredoxin Proteome Redoxome 
ProteomicsRedox-proteomic analysis of pancreatic β-cells upon glucose stimulation
Redox signaling mediated by reversible oxidative cysteine thiol modifications is crucial for driving cellular adaptation to dynamic environmental changes, maintaining homeostasis, and ensuring proper function. This is particularly critical in pancreatic β-cells, which are highly metabolically active...
ORGANISM(S): Mus musculus (Mouse) Rattus norvegicus (Rat) 
2024-09-30 | PXD052765 | Pride
Iodotmt Redox proteomics 
ProteomicsPeroxiredoxin 6 protects irradiated cells from oxidative stress and shapes their senescence-associated cytokine landscape, redox proteomic analysis
Cellular senescence is a complex stress response defined as an essentially irreversible cell cycle arrest mediated by the inhibition of cell cycle-specific cyclin dependent kinases. The imbalance in redox homeostasis and oxidative stress have been repeatedly observed as one of the hallmarks of the s...
ORGANISM(S): Homo sapiens (Human) 
2022-02-16 | PXD028780 | Pride
Peroxiredoxin 6 Cellular senescence Silac-iodotmt Ionizing radiation Redox proteomics 
ProteomicsRedox proteomics analysis of Arabidopsis wild type and grxs12 mutants
For an overall evaluation of the protein redox status in grxs12 leaves, the iodoTMT-based redox proteomics approach was employed. After protein extraction, the free thiols in Cys were NEM-blocked, and then the Cys thiols with reversible oxidations such as sulfenic acids (-SOH), S-glutathionylation (...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-01-16 | PXD059068 | Pride
Arabidopsis Redox proteomics Grxs12 mutants Iodotmt label 
ProteomicsCysteine Redox proteomics of PDIA6 over-expressing skeletal muscle
To investigate the role of PDIA6 on changes to specific cysteine residues with altered redox status and insulin senstivity, we overexpressed either GFP control or FLAG-PDIA6 throughout the musculature of 4-day old male C57BL/6J mice via intraperitoneal injection with MyoAAV (Fig. 5F). At 8 weeks of ...
ORGANISM(S): Mus musculus (Mouse) 
2026-05-29 | PXD075204 | Pride
Pdia6 Iodo-tmt 
ProteomicsRedox proteomics in NO-mediated protection on hypoxic cardiomyocyte
We developed a mass spectrometry (MS)-based quantitative approach using a set of novel iodoacetyl-based cysteine-reactive isobaric tags (iodoTMT) endowed with unique irreversible Cys-reactivities to differentially quantify the multiple redox-modified forms of a Cys site in the original cellular cont...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-06-27 | PXD000274 | Pride
S-nitrosylation Mass spectrometry Hypoxia Redox cysteine modifications Redox-proteomics 
ProteomicsS-nitrosoproteome analysis of rice leaves in response to Magnaporthe oryzae infection
S-nitrosoproteome of leaves obtained from two rice cultivars Dongjin (relatively resistant) and Nipponbare (susceptible) to Magnaporthe oryzae strain PO6-6 was investigated using a iodo-TMT six plex kit. MS/MS data was acquired using two MS intruments: QExactive™ Orbitrap High-Resolution Mass Spectr...
ORGANISM(S): Oryza sativa (Rice) 
2025-12-20 | PXD059677 | Pride
Rice Post-translational modifications Blast disease S-nitrosylation Pathogen 
ProteomicsWe applied an iodoacetyl tandem mass tags (iodoTMT)-based proteomic approach to analyze the redox proteome of Brassica napus seedlings under control and salt-stressed conditions. We identified 1,821 sulfenylated sites in 912 proteins from all samples.
By means of large-scale redox proteomics, we studied reversible cysteine modification during the response to short-term salt stress in Brassica napus. We applied an iodoacetyl tandem mass tags (iodoTMT)-based proteomic approach to analyze the redox proteome of Brassica napus seedlings under control ...
ORGANISM(S): Brassica napus (Rape) 
2021-08-18 | PXD027721 | Pride
Brassica napus Leaf Lc-ms/ms 
ProteomicsiodoTMT 6plex analysis of cysteine oxidation following eccentric contractions
Two iodoTMT 6-plex screens were used to assess the changes in protein cysteine oxidation following eccentric contractions in extensor digitorum longus (EDL) muscles that were extracted from wild type and mdx mice and treated with and without 750uM sodium hydrogen sulfide (NaHS). N=5 for each group: ...
ORGANISM(S): Mus <mouse, Genus> (ncbitaxon:10088) 
MSV000092827 | MassIVE
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