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Evaluation of mass spectrometry MS/MS spectra for the presence of isopeptide crosslinked peptides

Isopeptide crosslinked proteins can be the product of transglutaminase or of exposure to organophosphorus toxicants (OP). Transglutaminase links glutamine to lysine with loss of ammonia. OP toxicants induce a link between glutamic acid and lysine with loss of water. Our goal was to establish crit...

ORGANISM(S): Sus scrofa domesticus (domestic pig)

2021-07-12 | PXD026790 | Pride

Novel asymmetric isopeptide bond steers directional genomic RNA egress from icosahedral virus

How essential asymmetries are built into icosahedral virus to facilitate directional genome egress is unknown. Here we capture the structure and dynamics of disassembly intermediates of an icosahedral virus. We reveal localized RNA density poised for release beneath 5-fold axes on one half of the vi...

ORGANISM(S): Turnip crinkle virus

2025-11-06 | PXD067878 | Pride

Turnip crinkle virus Hdxms Rna egress Asymmetry Isopeptide bond

Dichlorvos-induced formation of isopeptide crosslinks between proteins in SH-SY5Y cells

Chlorpyrifos oxon catalyzes the crosslinking of proteins via an isopeptide bond between lysine and glutamic acid or aspartic acid in studies with purified proteins. Our goal was to determine the crosslinking activity of the organophosphorus pesticide, dichlorvos. We developed a protocol for examini...

ORGANISM(S): Homo sapiens (Human)

2023-10-24 | PXD033593 | Pride

Shsy5y human neuroblastoma cells Dichlorvos Mass spectrometry Manual evaluation Protein prospector Crosslink Isopeptide Crosslinks

Isopeptide-forming domain-containing fimbrial protein

Sequence analysis of an isopeptide-forming domain-containing fimbrial protein

PRJEB97399 | ENA

A global survey of intramolecular isopeptide bonds.

Not available

S-EPMC12617251 | biostudies-literature

Synthesis of isopeptide epoxide peptidomimetics.

Not available

S-EPMC2650424 | biostudies-literature