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ProteomicsQuantitative analysis of the supernatant from native and transfected CHO cells using iTRAQ 8-plex technique
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. A total...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2016-07-20 | PXD003462 | Pride
Cho supernatant Itraq 8-plex Lc-ms/ms 
ProteomicsComparative analysis of label-free and 8-plex iTRAQ approach for quantitative tissue proteomic analysis
MS-based approaches have been successfully utilized for the comprehensive characterization of the cell proteome. However, in the case of quantitative proteomics the open question still remains, which quantification method is more suitable for identification of proteins with the altered abundance, pa...
ORGANISM(S): Homo sapiens (Human) 
2015-09-09 | PXD002170 | Pride
Lc-ms/ms Bladder cancer Tissue proteomics Label-free Itraq 
UnknownPeptide labeling with isobaric tags yields higher identification rates using iTRAQ 4-plex compared to TMT 6-plex and iTRAQ 8-plex on LTQ Orbitrap.
Not available
S-EPMC3093924 | biostudies-literature
ProteomicsAnalysis of Reproducibility of Proteome Coverage and Quantitation Using Isobaric Mass Tags (iTRAQ and TMT)
This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex and TMT 6-plex reagents.
ORGANISM(S): Phaeosphaeria Nodorum Sn15 (ncbitaxon:321614) 
2016-03-17 | MSV000079594 | MassIVE
UnknownComparative Analysis of Label-Free and 8-Plex iTRAQ Approach for Quantitative Tissue Proteomic Analysis.
Not available
S-EPMC4557910 | biostudies-literature
ProteomicsComparative endometrial proteomics among fertile women, intrauterine device carriers, and recurrent implantation failure patients using iTRAQ methodology
Endometrial receptivity is imperative to achieving pregnancy in humans. A disruption in the development of endometrial receptivity is responsible for recurrent implantation failures (RIF) of endometrial origin. To further understand the molecular mechanisms behind the endometrial receptivity process...
ORGANISM(S): Homo sapiens (Human) 
2019-11-06 | PXD011094 | Pride
Intrauterine device (iud) Isobaric tags for relative and absolute quantification (itraq) Recurrent implantation failure (rif) Endometrial receptivity Endometrium 
ProteomicsDifferential regulation of tobacco host factors upon TMV infection
Tobacco mosaic virus (TMV) became one of the model virus for fundamental biological research and understanding of the host-pathogen interaction. It utilizes host machinery smartly to complete the life cycle, though many parts of life cycle remained a mystery, such as host factors involved in virus r...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2018-11-07 | PXD008837 | Pride
Tobacco Tmv Itraq 
UnknownDevelopment and characterization of novel 8-plex DiLeu isobaric labels for quantitative proteomics and peptidomics.
Not available
S-EPMC4837894 | biostudies-literature
UnknownQuantitative proteomic analysis of human embryonic stem cell differentiation by 8-plex iTRAQ labelling.
Not available
S-EPMC3377673 | biostudies-literature
ProteomicsRoot iTRAQ protein profile analysis of two Citrus species differing in aluminum-tolerance in response to long-term aluminum-toxicity
After long-term aluminum treatment, thereafter, we investigated Citrus root protein profiles using isobaric tags for relative and absolute quantification (iTRAQ).
ORGANISM(S): Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis) Citrus maxima (Pomelo) (Citrus grandis) 
2017-10-26 | PXD002301 | Pride
Citrus sinensis Aluminum Itraq Proteomic analysis 
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