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ProteomicsTalin1-GFP pulldown in U2OS cells
In this study, we pull-down human Talin1-GFP and GFP from U2OS cells plated on fibronectin. Binding partners were then analysed using mass-spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2021-05-12 | PXD024634 | Pride
Talin1 Gfp trap U2os 
ProteomicsAnalysis of Plasma Membrane, Endosomal and Cytoplasmic fractions of mouse embryonic fibroblast
This project aimed to identify novel endosomal proteins and therefore cellular fractionation experiments were performed. Adherent mouse embryonic fibroblast were scraped on ice into hypotonic lysis buffer (10 mM HEPES-KOH pH 7.2, 0.25 M sucrose, 1 mM EDTA, 1 mM MgOAc and protease & phosphatase inhib...
ORGANISM(S): Mus musculus (Mouse) 
2015-10-14 | PXD001870 | Pride
Cytosol Mouse embryonic fibroblasts Endosomes Cell fractionation Plasma membrane 
ProteomicsTLNRD1-GFP pulldown in HEK cells
In this study, we pull-down human TLNRD1-GFP and GFP from HEK cells. Binding partners were then analysed using mass-spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2024-01-27 | PXD045258 | Pride
Cell lines Gfp trap 
ProteomicsIdentification of novel SHARPIN binders
Sharpin, a multifunctional adaptor protein with oncogenic properties, regulates several signalling pathways. For example, Sharpin enhances signal-induced NF-κB signalling as part of the linear ubiquitin assembly complex (LUBAC) and inhibits integrins, the T cell receptor, caspase1 and PTEN. However,...
ORGANISM(S): Homo sapiens (Human) 
2017-08-11 | PXD004734 | Pride
Sharpin Gfp pull-down Human 
ProteomicsIdentification of Myosin-X (MYO10) proximal proteins using TurboID in U2-OS and U-87 MG cells
In this study, we aimed to investigate the composition of MYO10-positive filopodia and find novel MYO10 interactors using a proximity biotinylation method – TurboID in U2-OS and U-87 MG cells.
ORGANISM(S): Homo sapiens (Human) 
2025-12-30 | PXD067430 | Pride
ProteomicsProteomic analysis of integrin-associated adhesion complexes isolated from cells treated with DMSO or FAK inhibitor
Human foreskin fibroblast cells were spread on fibronectin (FN) for 1 h and treated with DMSO or FAK inhibitor (3 uM, AZ13256675 (termed AZ675 for short)) for 1 h. Integrin-associated adhesion complexes isolated from these cells were analysed by mass spectrometry to determine changes in adhesion com...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-08-23 | MSV000080089 | MassIVE
OtherIntegrin endosomal signalling suppresses anoikis.
Data from ProteomeXchange, PXD ID: PXD001870. Experiment: Plasma membrane fraction R2 raw, file: 150120_106.mzml. Published as part of Nat Cell Biol. 2015 Oct 5 . From the Abstract: {{i}} Integrin-containing focal adhesions transmit extracellular signals across the plasma membrane to modulate cell ...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32310025155 | GPMDB
Resource Reanalysis 
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