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ProteomicsAPEX2 proximity labeling of PI(3)P dependent ER and early endosome membrane contact tether proteins
To understand the nature of ER and early endosome membrane contact site, we capitalized the MTM1 knock out (KO) HeLa cells that do not dissociate the ER and early endosome membrane contact upon nutrient starvation. By using the Doxycyclin inducible APEX2 fused Rab5A (early endosome marker) expressin...
ORGANISM(S): Homo sapiens (Human) 
2023-01-06 | PXD033846 | Pride
Hela Apex2 Lc-ms Mtm1 Early endosome Er Membrane contact 
ProteomicsQuantitative proteomics of nutrient starved wildtype (WT) and MTM1 knock out (KO) HeLa cells.
Quantitative whole cell proteomics that compare fed or starved wildtype (WT) and MTM1 KO HeLa cells.
ORGANISM(S): Homo sapiens (Human) 
2022-12-22 | PXD033850 | Pride
Lc-ms Starvation Hela Tmt-labeling Mtm1 
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