Sort   by:  
 Page size 
ProteomicsExploring Alternative Cysteine-reactive Chemistry for MS-cleavable Cross-linking Mass Spectrometry
To enable global PPI mapping, we have explored an alternative cysteine labeling chemistry, and thus designed and synthesized a sulfoxide-containing MS-cleavable haloacetamide-based cross-linker, DiBromoAcetamide SulfOxide (DBrASO). In combination with a newly developed SEC-HpHt fractionation method,...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD037443 | Pride
Cysteine Proteomics Cross-linking mass spectrometry 
ProteomicsDSBSO-based XL-MS Analysis of Breast Cancer PDX Tissues to Delineate Protein Interaction Network in Clinical Samples
We applied our previously established Alkyne-A-DSBSO-based in vivo XL-MS analytical platform on two types of breast cancer PDX samples cross-linking. Coupled with the LC-MSn strategy, the experiments enable us to obtain information on cancer-related protein-protein interactions since experimenting o...
ORGANISM(S): Homo sapiens (Human) 
2024-03-20 | PXD046966 | Pride
Breast cancer Pdx Cross-linking mass spectrometry 
ProteomicsCysteine-enabled Cleavability to Advance Cross-linking Mass Spectrometry for Global Analysis of Endogenous Protein-Protein Interactions
We have employed the sulfoxide-based cleavability to establish a novel cysteine-based MS-cleavable XL-MS platform, enabling proteome-wide PPI analysis using non-cleavable heterobifunctional K-C linkers. The effectiveness of the newly established platform has been demonstrated using three commerciall...
ORGANISM(S): Homo sapiens (Human) 
2025-10-17 | PXD055169 | Pride
Protein structure Cross-linking mass spectrometry Alphafold2 Cysteine 
ProteomicsStructural analysis of the CSN complex to understand its inhibition mechanism
We performed interaction and structural analyses to unravel the molecular mechanisms underlying CSN inhibition. Various methods including Cryo-EM, cross-linking mass spectrometry, quantitative mass spectrometry and biochemical assays have been employed to define inhibitor-induced interaction and str...
ORGANISM(S): Homo sapiens (Human) 
2025-11-20 | PXD063786 | Pride
Csn Quantitative ms Crls Protein ubiquitination Neddylation Xl-ms Cryo-em Cop9 signalsome 
Sort   by:  
 Page size