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ProteomicsAtopic dermatitis epidermis PCT-DIA
Atopic Dermatitis (AD) is the most common inflammatory skin disease and characterized by a deficient epidermal barrier and cutaneous inflammation. Genetic studies suggest a key role of keratinocytes in AD pathogenesis, but the alterations in the proteome that occur in the entire epidermis have not b...
ORGANISM(S): Homo sapiens (Human) 
2022-10-04 | PXD025431 | Pride
Pressure cycling technology Atopic dermatitis Data-independent acquisition Epidermis 
ProteomicsTendon tissue degradome in response to the patho-physiological niche
Aberrant matrix turnover with elevated matrix proteolysis is a hallmark of tendon pathology. While tendon disease mechanisms remain obscure, mechanical cues are central regulators. Unloading of tendon explants in standard culture conditions provokes rapid cell-mediated tissue breakdown. Here we show...
ORGANISM(S): Mus musculus (Mouse) 
2020-01-28 | PXD013635 | Pride
Mouse Tendon Thermo q exactive Tails 
ProteomicsTime-resolved substrate degradomics
20110318_GluC: Cell culture supernatants from Balb/c 3T3 mouse fibroblasts that had been incubated with the endoproteinase GluC (Staphylococcus aureus protease V8) as test protease for 1, 2, 4, 8, and 16 hours or buffer alone (0, 12, and 16 hours controls) were subjected to 8plex-iTRAQ-TAILS analys...
ORGANISM(S): Mus musculus (Mouse) 
2013-12-13 | PXD000503 | Pride
Protease 8plex-itraq Tails Kinetics Mmp 
ProteomicsMMP10 degradomics in keratinocytes
Matrix metalloproteinases (MMPs) are important players in skin homeostasis, wound repair, and in the pathogenesis of skin cancer. One member – MMP10 – is specifically expressed in wound keratinocytes and in epithelial cancer cells, but its function is unclear and epidermal substrates are poorly char...
ORGANISM(S): Mus musculus (Mouse) 
2015-10-19 | PXD002474 | Pride
Itraq-tails Keratinocytes Mmp10 Skin Degradomics 
ProteomicsIn Vivo Assessment of Protease Dynamics in Cutaneous Wound Healing by Degradomics Analysis of Porcine Wound Exudates
Proteases control complex tissue responses by modulating inflammation, cell proliferation and migration, and matrix remodeling. All these processes are orchestrated in cutaneous wound healing to restore the skin’s barrier function upon injury. Altered protease activity has been implicated in the p...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2014-12-19 | PXD001198 | Pride
N-terminome Wound healing Protease Tails Wound fluids 
ProteomicsComparative degradomics of porcine and human wound exudates unravels biomarker candidates for assessment of wound healing progression in trauma patients
Impaired cutaneous wound healing is a major complication in elderly people and patients suffering from diabetes with raising rates in industrialized countries. Heterogeneity of clinical manifestations hampers effective molecular diagnostics and decisions for appropriate therapeutic regimens. Using a...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2017-09-14 | PXD006674 | Pride
Impaired wound healing Negative pressure wound therapy Positional proteomics Tails Wound fluids Biomarker 
ProteomicsQuantitative proteomics analysis of lung tissue from E18.5 embryos with mesenchymal deletion of VLK
Vertebrate lonesome kinase (VLK) is the only known secreted tyrosine kinase and responsible for the phosphorylation of a broad range of secretory pathway-resident and extracellular matrix proteins. However, its cell-type specific functions in vivo are still largely unknown. Therefore, we generated m...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-11 | PXD037700 | Pride
Embryo Lung Vertrebrate lonesome kinase 
ProteomicsTAILS proteomics of murine pancreatic tumors
Aberrant proteolysis by cysteine cathepsins is implicated in carcinogenesis, but knowledge of cathepsin substrates mediating tumor-promoting or suppressing effects is limited. Here we characterize tumor proteome and in vivo cathepsin substrates using cathepsin knockout mice and the RIP1-Tag2 model o...
ORGANISM(S): Mus musculus (Mouse) 
2016-08-08 | PXD003637 | Pride
Pancreatic neuroendocrine cancer Ecm Proteomics Substrate discovery Proteolytic processing Degradation Proteases Tails n-terminomics Cysteine cathepsins 
ProteomicsQuantitative proteomics of barrier-deficient mouse epidermis in response to environmental humidity
Low environmental humidity aggravates symptoms of inflammatory skin diseases, e.g. of Atopic Dermatitis (AD). Using mice that develop AD-like symptoms, we show that an increase in environmental humidity rapidly rescues their cutaneous inflammation and associated epidermal abnormalities. Quantitative...
ORGANISM(S): Mus musculus (Mouse) 
2018-05-14 | PXD007124 | Pride
Mouse Skin Barrier defect Humidity Epidermis Osmoregulation 
UnknownSubstrate O-glycosylation actively regulates extracellular proteolysis.
Not available
S-EPMC11285871 | biostudies-literature
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