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ProteomicsActivity-Guided Proteomic Profiling of Proteasomes - Whole proteome studies
A composition of proteasome complexes in WT yeast, and the changes these complexes undergo upon the deletion of Pre9 (Δα3) or of Sem1 (ΔSem1) based on whole-cell proteomic analysis and to activity-guided proteasome profiling of indicates that the amounts of proteasomal proteins and proteasome intera...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-02-15 | PXD045047 | Pride
Activity-guided profilling Proteasome 
ProteomicsActivity-Guided Proteomic Profiling of Proteasomes
A composition of proteasome complexes in WT yeast, and the changes these complexes undergo upon the deletion of Pre9 (Δα3) or of Sem1 (ΔSem1) based on whole-cell proteomic analysis and to activity-guided proteasome profiling of indicates that the amounts of proteasomal proteins and proteasome intera...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-02-15 | PXD045051 | Pride
Activity-guided profilling Proteasome 
ProteomicsActivity guided profiling of yeast proteasome - protein extraction test
This experiment compared the impact of protein extraction conditions on the identification and quantification of proteasome subunits in WT yeast. It involved comparing proteins extracted from identical WT yeast samples under two different conditions: native and denaturing (in the presence of SDS and...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-02-15 | PXD048380 | Pride
Native extraction Proteasome Yeast lfq 
ProteomicsTime-resolved N-terminome analysis of HCT116 cells during apoptosis
Proteins' N-termini contain information about their biochemical properties and functions, and they can undergo co- or post-translational modifications, as well as be processed by proteases. To expand the coverage of the N-terminome, we have developed LATE (LysN Amino Terminal Enrichment), a method t...
ORGANISM(S): Homo sapiens (Human) 
2023-06-27 | PXD040732 | Pride
Caspase-3 N-terminal acetylation N-terminome Apoptosis 
ProteomicsCell-based characterization of caspase-3 cleavage sites by LATE and HYATNE
Proteins' N-termini contain information about their biochemical properties and functions, and they can undergo co- or post-translational modifications, as well as be processed by proteases. To expand the coverage of the N-terminome, we have developed LATE (LysN Amino Terminal Enrichment), a method t...
ORGANISM(S): Homo sapiens (Human) 
2023-06-27 | PXD036648 | Pride
Late Hytane Abt199 Lysn Peptidyl-lys metalloendopeptidase n-terminomics Caspase-3 
ProteomicsIn vitro characterization of caspase-3 cleavage sites by LATE and HYATNE
Proteins' N-termini contain information about their biochemical properties and functions, and they can undergo co- or post-translational modifications, as well as be processed by proteases. To expand the coverage of the N-terminome, we have developed LATE (LysN Amino Terminal Enrichment), a method t...
ORGANISM(S): Homo sapiens (Human) 
2023-06-27 | PXD036593 | Pride
Late Hytane Lysn Peptidyl-lys metalloendopeptidase n-terminomics Caspase-3 
ProteomicsInfection cycle and phylogeny of a Polinton-like-virus virophage infecting Phaeocystis globosa
Virophages are small dsDNA viruses dependent on a nucleocytoplasmic large-DNA virus infection of a cellular host for replication. Putative virophages infecting algal hosts are classified together with Polinton-like viruses, transposable elements widely found in algal genomes, yet the lack of isolate...
ORGANISM(S): Phaeocystis globosa virus virophage Phaeocystis globosa virus Phaeocystis globosa Phaeocystis globosa virus 14T 
2023-02-11 | PXD036892 | Pride
Polinton-like-virus virophage Phaeocystis globosa 
ProteomicsMapping the DPP9 Interactome by Proximity Labeling
Dipeptidyl peptidase 9 (DPP9) is an amino peptidase with the unusual ability to cleave a peptide bond post-proline. Its function affects immunity, DNA-repair, cell signaling, memory and neonatal survival; its dysregulation is linked to cancer and immune-related disorders. While most studies focus on...
ORGANISM(S): Homo sapiens (Human) 
2026-08-31 | PXD067345 | Pride
Proximity labeling Dpp9 Turboid 
ProteomicsSignature cleavage in vitro assay of 20S and 26S proteasome using naked and ubiquitined cyclin
A systematic and comparative study of the proteolysis products generated by purified human 26S and 20S proteasome following the in vitro cleavage of non-modified (naked), mono-ubiquitinated and poly-ubiquitinated cyclin B.
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD018711 | Pride
Human 26s proteasome 20s proteasome Cyclin 
ProteomicsSignature activities of 20S proteasome reveal degradation of intact ubiquitin-conjugates in hypoxia
Careful removal of unwanted proteins is necessary for cell survival. The primary constitutive intracellular protease is the 26S proteasome complex, very often found in equilibrium with its catalytic core particle – the 20S subcomplex. Protein degradation by the former is tightly regulated due to pri...
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD018722 | Pride
26s 20s Peptides Proteasome Hypoxia 
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