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The in vivo labeling technique Split-Biotin identification (Split-BioID, De Munter et al., 2017, Schopp et al., 2017, Cho et al., 2020) was used to capture the common microenvironment of Bre5 and the ribosomal protein Rps2 (uS5). Biotinylated proteins were enriched from cell lysates using affinity p...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-11-10 | PXD028879 | Pride
The object of this project is to investigate the proximal proteome of ULK1 during mitophagy and the effects of a FIP200 knock down using the APEX2-mediated proximity labeling system. APEX2-ULK1 or APEX2 was fused to 2xFKBP-myc and stably expressed in a cell line stably expressing a truncated FIS1-FR...
ORGANISM(S): Homo sapiens (Human) 
2024-10-12 | PXD047277 | Pride
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