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Neatly every proteomic protocol has a Cysteine reduction and alkylation step in it preceding the trypsin digestion. It needs to be done in order to remove the s-s bonds and prevent proteins from folding, thus, provide better access for trypsin. Among various reagents used for alkylation, iodoacetami...
ORGANISM(S): Homo sapiens (Human) 
2020-10-28 | PXD013973 | Pride
Neatly every proteomic protocol has a Cysteine reduction and alkylation step in it preceding the trypsin digestion. It needs to be done in order to remove the s-s bonds and prevent proteins from folding, thus, provide better access for trypsin. Among various reagents used for alkylation, iodoacetami...
ORGANISM(S): Homo sapiens (Human) 
2024-02-14 | PXD015889 | Pride
This dataset is a part of a bigger work on RNA editing in D. melanogaster brain. We present Orbitrap spectra searched against a customized database of Drosophila proteome with introduction of variants provided by RNA editing. Three files reflect 3 technical replicates.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2018-10-25 | PXD009590 | Pride
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