Stable cell line was used for this study, so there are minimum variations between samples. For the lable free based quantitative analysis of the whole proteome, three biological replicates for each condition were investigated. For the phosphoproteomic analysis, three biological replicates and two te...
In order to identify sites of the SEC16A specifically phosphorylated by the Dual Specificity Kinase DYRK3, mCherry-SEC16A was transiently over expressed in HeLa cells together with GFP-DYRK3 or EGFP-C1 only
Even though these results have shown several key proteins played an import role in radiation tolerance mechanism of DR, they were lack of the deep protein coverage, global protein analysis as well as the dynamic changes of DR, which limits further understanding of biological changes in response to i...
To improve preservation quality of sweet cherry (Prunus avium L.), the effect of ozone (O3) was estimated by label-free quantification proteomics and weighted gene co-expression network analysis (WGCNA).
This study provides a comprehensive proteomic analysis of five matched pairs of the anterior and posterior lobe of the pituitary, which was used to study the proteomic signature specific to both anterior and posterior lobes. Anterior Lobe n=5 Posterior Lobe n=5 A441 PT441 A442 ...
APEX1 overexpression and knockdown cell lines were established based on SKOV3 cell line. Lable-free quantitative phosphoproteomics was done to evaluate the impact of APEX1 on cellular phosphoproteomics
Oil body (OB) is an important intracellular organelle for triacylglycerols (TAGs) storage. In present study, OB fractions from three developmental stages of Chinese hickory (Carya cathayensis) seed were purified and the OB fraction accumulated proteins were then isolated and analyzed by liquid chrom...
Pepper(Capsicum annuum L.) fruit development is a complex and genetically programmed process, a comparative study of transcriptome and proteome changes during two varieties of pepper development（IMG, MG, Br and MR） has been carried out by using RNA-Seq and Lable-free quantitation technology.
