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ProteomicsAge-related changes in protein abundance in nematodes
Using quantitative mass spectrometry together with protein and peptide-level fractionation, we can now report a major increase in coverage of the C. elegans proteome, identifying over 9,300 proteins in three biological replicates. The resulting data have been assembled into a searchable, online reso...
ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239) 
2015-08-21 | MSV000079263 | MassIVE
ProteomicsProteomic Analysis of the Response to Cell Cycle Arrests in Human Myeloid Leukemia Cells
Previously (Ly et al. 2014), we analyzed protein abundance changes across a ‘minimally perturbed’ cell cycle in human cells (NB4), using centrifugal elutriation to differentially enrich distinct cell cycle phases. Here, we compare data from elutriated cells with NB4 cells arrested at comparable ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080841 | MassIVE
ProteomicsHs_GlobalSubcellular_SILAC
Characterisation of PRR11
ORGANISM(S): Homo Sapiens (human) 
2013-12-31 | PAe005033 | PeptideAtlas
ProteomicsGlobal Subcellular Characterization of Protein Degradation Using Quantitative Proteomics
Protein degradation provides an important regulatory mechanism used to control cell cycle progression and many other cellular pathways. To comprehensively analyze the spatial control of protein degradation in U2OS osteosarcoma cells, we have combined drug treatment and SILAC-based quantitative mass ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080816 | MassIVE
ProteomicsGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-08-02 | MSV000080014 | MassIVE
ProteomicsEnhanced snoMEN vectors facilitate establishment of GFP–HIF-1? protein replacement human cell lines
The snoMEN (snoRNA Modulator of gene ExpressioN) vector technology was developed from a human box C/D snoRNA, HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets, allowing knock-down of targeted genes. Here we have screened additional human ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080828 | MassIVE
ProteomicsPlant SILAC: Stable-Isotope Labelling with Amino Acids of Arabidopsis Seedlings for Quantitative Proteomics.
Stable Isotope Labelling by Amino acids in Cell culture (SILAC) is a powerful technique for comparative quantitative proteomics, which has recently been applied to a number of different eukaryotic organisms. Inefficient incorporation of labelled amino acids in cell cultures of Arabidopsis thaliana h...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2016-08-15 | MSV000080043 | MassIVE
ProteomicsHipSci: the iPSC proteomic compendium
Human induced pluripotent stem cell (iPSC) technology holds great potential for therapeutic and research purposes, by providing improved models of disease and for studying mechanisms regulating differentiation. The Human Induced Pluripotent Stem Cell Initiative (HipSci) was established to generate l...
ORGANISM(S): Homo sapiens (Human) 
2019-07-25 | PXD010557 | Pride
Dda Ipsc Proteomics Homo sapiens Human Orbitrap Fusion Stem cells Tmt 
ProteomicsTissue adaptation is the dominant driver of the proteomic landscape of intestinal intraepithelial lymphocytes
Intestinal intraepithelial T lymphocytes (T-IEL) patrol the single layer of epithelial cells lining the gut, and consist of both induced T-IEL, derived from systemic antigen-experienced lymphocytes, and natural IEL, that are developmentally targeted to the intestine. To gain functional insights into...
ORGANISM(S): Mus musculus (Mouse) 
2021-03-15 | PXD023140 | Pride
Tmt T cells Lymph nodes Lymphocytes Iel 
ProteomicsLaser-capture proteomics of human FFPE substantia nigra
Laser-capture microdissection (LCM) allows the visualization and isolation of morphologically distinct subpopulations of cells from heterogeneous tissue specimens. In combination with formalin-fixed and paraffin-embedded (FFPE) tissue it provides a powerful tool for retrospective and clinically rele...
ORGANISM(S): Homo sapiens (Human) 
2020-03-05 | PXD016563 | Pride
Laser-capture microdissection Tmt Ffpe Substantia nigra 
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