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ProteomicsCryoEM structure of mitochondrial complex I from the thermophilic eukaryote Chaetomium thermophilum
Mitochondrial complex I is a redox-driven proton pump that generates most of the proton-motive force powering oxidative phosphorylation and ATP synthesis in eukaryotes. We report the structure of complex I from the thermophilic eukaryote Chaetomium thermophilum, determined by electron cryo-microscop...
ORGANISM(S): Chaetomium thermophilum 
2022-11-29 | PXD033234 | Pride
Mitochondrial complex i Chaetomium thermophilum 
ProteomicsIsolation of a novel heterodimeric PSII complex involved in PSII repair
The multi-subunit membrane protein complex Photosystem II (PSII) catalyzes the light driven oxidation of water and with this the initial step of photosynthetic electron transport in plants, algae, and cyanobacteria. Its biogenesis is coordinated by a network of auxiliary proteins that facilitate the...
ORGANISM(S): Thermosynechococcus elongatus BP-1 
2023-02-06 | PXD033676 | Pride
Cyanobacteria Psbo Thermosynechococcus elongatus Photosynthesis Photosystem ii 
ProteomicsRotary substates of mitochondrial ATP synthase reveal the basis of flexible F1-FO coupling
F1FO-ATP synthases play a central role in cellular metabolism, making the energy of the protonmotive force across a membrane available for a large number of energy-consuming processes. We determined the single-particle cryo-EM structure of active dimeric ATP synthase from mitochondria of Polytomella...
ORGANISM(S): Polytomella parva 
2019-05-01 | PXD013530 | Pride
Proteomics Atp synthase Electron microscopy Polytomella 
ProteomicsDissecting the RAD51–BRC4 Interaction Landscape through Integrative Molecular Simulations and Experimental Biophysics
The interaction between the RAD51 and BRCA2 proteins is central to homologous recombination (HR), a crucial pathway ensuring high-fidelity DNA repair. Recruitment of RAD51 involves eight highly conserved regions on BRCA2, named BRC repeats. The interaction between the fourth BRC repeat (BRC4) and th...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-10-29 | PXD060867 | Pride
ProteomicsCysteine oxidation and disulfide formation in the ribosomal exit tunnel
Understanding the conformational sampling of translation-arrested ribosome nascent chain complexes is key to understand co-translational folding. Up to now, coupling of cysteine oxidation, disulfide bond formation and structure formation in nascent chains has remained elusive. Here, we investigate t...
ORGANISM(S): Bos taurus (Bovine) Escherichia coli 
2020-11-09 | PXD021574 | Pride
Glutathione Ribosomal exit tunnel Cysterine oxidation Disulfide formation 
ProteomicsLigand binding and conformational dynamics of the E. coli nicotinamide nucleotide transhydrogenase revealed by hydrogen/deuterium exchange mass spectrometry
Nicotinamide nucleotide transhydrogenases are integral membrane proteins that utilizes the proton motive force to reduce NADP+ to NADPH while converting NADH to NAD+. Atomic structures of various transhydrogenases in different ligand-bound states have become available, and it is clear that the molec...
ORGANISM(S): Escherichia coli 
2022-10-26 | PXD037413 | Pride
Channel opening Membrane protein Small ligand binding Hdx-ms Cyclic ims‑ms 
ProteomicsAn integrated transcriptomic and proteomic map of the mouse hippocampus at synaptic resolution
Understanding the brain’s molecular diversity requires spatially resolved maps of transcripts and proteins across regions and compartments. Here, we performed deep spatial molecular profiling of the mouse hippocampus, combining microdissection of 3 subregions and 4 strata with fluorescence-activated...
ORGANISM(S): Mus musculus (Mouse) 
2025-08-28 | PXD054879 | Pride
Proteomics Hippocampus Dia Synaptosome Brain Ca1 Transcriptomics 
ProteomicsCo-translational assembly counteracts promiscuous interactions
During the co-translational assembly of protein complexes, a fully synthesized subunit engages with the nascent chain of a newly synthesized interaction partner. Such events are thought to contribute to productive assembly, but their exact physiological relevance remains underexplored. Here, we exam...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-11 | PXD028413 | Pride
Protein complex assembly Co-translational assembly Nuclear pore complex 
ProteomicsCo-translational assembly counteracts promiscuous interactions
During the co-translational assembly of protein complexes, a fully synthesized subunit engages with the nascent chain of a newly synthesized interaction partner. Such events are thought to contribute to productive assembly, but their exact physiological relevance remains underexplored. Here, we exam...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-11 | PXD030626 | Pride
Co-translational assembly Proteomics 
ProteomicsINTEGRATED MULTI-OMICS ANALYSIS OF PBX1 IN MOUSE ADULT NEURAL STEMAND PROGENITOR CELLS IDENTIFIES A TRANSCRIPTIONAL MODULE WITH RELEVANCE TO LEUKEMOGENESIS
Developmental transcription factors act in networks, but how these networks achieve cell- and tissue specificity is still poorly understood. We here explored pre-B-cell leukemia homeobox 1 (PBX1) in adult neurogenesis combining genomic, transcriptomic, and proteomic approaches. ChIP-Seq analysis unc...
ORGANISM(S): Mus musculus (Mouse) 
2024-09-16 | PXD048222 | Pride
Multi-omics Pbx1 Leukemogenesis 
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