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UnknownA multiplexed microflow LC-MS/MS-PRM assay for serologic quantification of IgG N- and HPX O- glycoforms in liver fibrosis.
Not available
S-EPMC9837073 | biostudies-literature
UnknownQuantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS.
Not available
S-EPMC7373126 | biostudies-literature
ProteomicsLC-MS/MS-PRM Quantification of IgG glycoforms using stable isotope labeled IgG1 Fc glycopeptide standard
LC-MS/MS-PRM Quantification of IgG glycoforms using stable isotope labeled IgG1 Fc glycopeptide standard
ORGANISM(S): Homo Sapiens (human) 
2024-01-16 | PXD039462 | JPOST Repository
ProteomicsA multiplexed microflow LC-MS/MS-PRM assay for serologic quantification of IgG N- and HPX O- glycoforms in liver fibrosis
In this study, we introduce a targeted microflow LC-MS/MS-PRM method for the quantification of multiple glycopeptides in unfractionated serum samples. The entire preparation of 16 samples in a batch is completed within 3 h, and the LC-MS quantification of all the glycoforms in a sample is completed ...
ORGANISM(S): Homo Sapiens (human) 
2023-10-25 | PXD037687 | JPOST Repository
ProteomicsThe parallel reaction monitoring (PRM) target sequencing based on the comparative proteomic profiling in sheep spermatozoa under non-capacitating (NC) and capacitating (CAP) conditions in vitro using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with tandem mass tag (TMT) labeling strategy
To verify protein expression levels obtained by TMT LC-MS/MS analysis strategy, the expression levels of differentially abundant proteins in NC and CAP groups were selected and quantified by a PRM assay.
ORGANISM(S): Bos taurus (Bovine) 
2022-07-14 | PXD034330 | Pride
Spermatozoa Prm Tmt Proteomic Lc-ms/ms Capacitation 
ProteomicsTargeted LC-MS/MS analysis of oSCRIB protein in HeLa cells.
LC-MS/MS analysis of small HeLa proteins (approximately 10–15 kDa) for detecting oSCRIB protein with Parallel Reaction Monitoring (PRM) mode.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027841 | Pride
Human Prm Hela Lc-ms/ms 
ProteomicsProteomic profiling and PRM analysis for quantification of heterologous antigens in HIV-1 Gag VLPs
In this study, we evaluated HIV-1 Gag Virus Like Particles (VLPs) for the display of heterologous membrane proteins in their native conformation and lipid environment. The overexpressed membrane protein was quantified in the VLPs by LC-MS/MS based PRM analysis.
ORGANISM(S): Homo sapiens (Human) 
2026-02-20 | PXD074653 | Pride
Vlps Prm Lc-ms/ms 
ProteomicsPRM-MS quantitative analysis of isomeric N-glycopeptides derived from human serum haptoglobin of patients with cirrhosis and hepatocellular carcinoma
Comprehensive LC-PRM-MS approach where a targeted parallel reaction monitoring (PRM) strategy was coupled to a powerful LC system to study the microheterogeneity of serum haptoglobin (Hp) extracted from 15 patients with cirrhosis and 15 with hepatocellular carcinoma (HCC).
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD023719 | Pride
N-glycopeptides Prm Hepatocellular carcinoma (hcc) Haptoglobin Cirrhosis 
UnknownA Rapid LC-MS/MS-PRM Assay for Serologic Quantification of Sialylated O-HPX Glycoforms in Patients with Liver Fibrosis.
Not available
S-EPMC9000230 | biostudies-literature
ProteomicsA quick guideline for PRM method development applied to target proteomics in biological fluids
Combining in-silico analysis, serum high abundant protein depletion, and DDA and PRM acquisitions, we established a simple workflow to select PRM high response peptides for protein quantification in serum. The workflow enables evaluating peptide intensity, MS2 match with the spectral library, peptid...
ORGANISM(S): Homo sapiens (Human) 
2024-02-05 | PXD045089 | Pride
Complement system Prm Human serum Quantitative proteomics Lc-ms/ms 
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