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ProteomicsBLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures
We ran native 5% polyacrylamide-TBE gel for electro mobility shift assay (EMSA) for DNA/RNA G4 structures with or without recombinant human core-BLM (cBLM) protein, which was extracted and purified from E.coli in order to show binding of the RNA/DNA by this protein. To further evidence, we cut the e...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD042983 | Pride
Blm Sg Emsa G-quadruplex Lc-ms/ms 
ProteomicsBLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures
Bloom’s syndrome (BLM) protein is a known nuclear helicase that is able to unwind DNA secondary structures such as G-quadruplexes. However, its role in the regulation of cytoplasmic processes that involve RNA G-quadruplexes (rG4s) has not been previously studied. Here, we demonstrate that BLM is rec...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039534 | Pride
Stress granule Apex Helicase Lc-msms G-quadruplex Rg4-binding proteins Blm Quma-1 
ProteomicsSimultaneous metagenome-informed metaproteomics of the human gut microbiome, host and dietary exposome uncover functional interactomes in health & IBD
Host-microbiome-dietary interactions play crucial roles in regulating human health, yet direct functional assessment of their interplays, cross-regulations and downstream disease impacts remains challenging. We adopted metagenome-informed metaproteomics (MIM), in both mice and humans, to simultaneou...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) gut metagenome 
2025-08-08 | PXD057701 | Pride
Microbiome Diet Metagenomics-informed metaproteomics Proteomics Host Biomarker 
TranscriptomicsInferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters
Despite extensive research, our understanding of the rules according to which cis-regulatory sequences are converted into gene expression is limited We devised a method for obtaining parallel, highly accurate gene expression measurements from thousands of designed promoters and applied it to measure...
ORGANISM(S): Saccharomyces cerevisiae 
2012-05-20 | E-GEOD-37851 | biostudies-arrayexpress
TranscriptomicsProbing the effect of promoters on noise in gene expression using thousands of designed sequences
Genetically identical cells exhibit large variability (noise) in gene expression, with important consequences for cellular function. Although the amount of noise decreases with and is thus partly determined by the mean expression level, the extent to which different promoter sequences can deviate aw...
ORGANISM(S): synthetic construct 
2014-07-12 | E-GEOD-55346 | biostudies-arrayexpress
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