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ProteomicsLYSET/TMEM251 SILAC Co-IP from MIA PaCA-2
Endogenous LYSET/TMEM251 was isolated via Co-IP from MIA PaCa-2 cells. LYSET KO cells were used as controls. Cells were grown in SILAC medium for 3 passages
ORGANISM(S): Homo sapiens (Human) 
2022-09-29 | PXD035821 | Pride
Silac co-ip Human Lyset Tmem251 
ProteomicsLYSET/TMEM251 KO and GNPTAB KO secretomes from MEFs
Analysis of proteins secreted over 24 h in OptiMEM by LYSET/TMEM251 knockout, GNPTAB knockout and wild-type MEFs
ORGANISM(S): Mus musculus (Mouse) 
2022-09-29 | PXD035822 | Pride
Mefs Lyset Secretome Tmem251 Gnptab 
UnknownThe human disease gene LYSET is essential for lysosomal enzyme transport and viral infection.
Not available
S-EPMC9547973 | biostudies-literature
UnknownGOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway.
Not available
S-EPMC11649813 | biostudies-literature
ProteomicsThe Human Disease Gene LYSET is Essential for Lysosomal Enzyme Transport and Viral Infection
Lysosomes are key degradative compartments of the cell. Transport to lysosomes ismediated by tagging soluble enzymes with mannose 6-phosphate (M6P) by GlcNAc-1-phosphotransferase whose deficiency leads to the severe lysosomal storage disorder mucolipidosisII (MLII). Several viruses require lysosomal...
ORGANISM(S): Mus musculus (Mouse) 
2022-09-15 | PXD029609 | Pride
Data independent acquisition (dia) Tmem 251 Parallel reaction monitoring (prm) Targeted ms Label free quantification Mouse embryonic fibroblasts 
ProteomicsLYSET/TMEM251 KO secretome from MIA PaCa-2 cells
Analysis of proteins secreted over 24 h in OptiMEM by LYSET/TMEM251 knockout and control MIA PaCa-2 cells.
ORGANISM(S): Homo sapiens (Human) 
2022-09-29 | PXD029658 | Pride
Human Pancreatic cancer Secretome 
ProteomicsGOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway
Glycosylation plays an important role for modifications of lipids and sorting of proteins that is regulated by asymmetric intra-Golgi distribution and SPPL3-mediated cleavage of Golgi enzymes. We found that cells lacking the Golgi N-acetylglucosamine phosphotransferase (GNPT) retention factor LYSET/...
ORGANISM(S): Homo sapiens (Human) 
2024-11-27 | PXD052083 | Pride
Golgi apparatus  lysosomes  mannose 6-phophate tagging  glycosyltransferase secretion  intramembrane proteolysis  copi vesicles 
ProteomicsLYSET/TMEM251 KO cellular and lysosomal proteomes from MIA PaCa-2 cells
Proteome analysis of post-nuclear supernatants (PNS) and enriched lysosomal fractions from LYSET/TMEM251 knockout and control MIA PaCa-2 cells. Lysosomes were loaded with iron nanoparticles (DexoMAG C, Liquids Research). PNS were generated from cell homogenates and used as input to enrich lysosomal...
ORGANISM(S): Homo sapiens (Human) 
2022-09-29 | PXD029659 | Pride
Pns Lysosomes Human Pancreatic cancer 
UnknownFunctional enrichment analysis of LYSET and identification of related hub gene signatures as novel biomarkers to predict prognosis and immune infiltration status of clear cell renal cell carcinoma.
Not available
S-EPMC10645642 | biostudies-literature
ProteomicsPeroxisome-derived ether lipids regulate lysosomal homeostasis through exocytosis control
Lysosomes and peroxisomes are essential for cellular homeostasis, yet how their activities are coordinated remains poorly understood. Here, we identify peroxisome-derived ether lipids as key regulators of lysosomal function. A genome-wide CRISPR/Cas9 screen in Mucolipidosis V (LYSET-deficient) cells...
ORGANISM(S): Homo sapiens (Human) 
2026-05-03 | PXD074652 | Pride
Human Lysosome Lyset Silac Ether-lipid Far1 
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