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ProteomicsIMPROVED N- AND O-GLYCOPEPTIDE IDENTIFICATION USING HIGH-FIELD ASYMMETRIC WAVEFORM ION MOBILITY SPECTROMETRY (FAIMS)
Mass spectrometry is the premier tool for identifying and quantifying site-specific protein glycosylation globally. Analysis of intact glycopeptides often requires an enrichment step, after which the samples remain highly complex and exhibit a broad dynamic range of abundance. Here, we evaluated th...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD038673 | Pride
Faims N-glycopeptides Mass spectrometry O-glycopeptides Glycoproteomics 
UnknownLocal externalization of phosphatidylserine mediates developmental synaptic pruning by microglia.
Not available
S-EPMC7429741 | biostudies-literature
ProteomicsEvaluation of a bead-free immunoprecipitation technique coupled with tandem mass spectrometry for identification of plant-virus protein interactions (DeBlasio_JBT_2017)
Potato leafroll virus (PLRV) is an aphid-borne, RNA virus in the Luteoviridae that causes significant loss to potato production worldwide. Precision methods to block virus infection in plants or the transmission of a virus by the insect represent new approaches to virus disease management and requir...
ORGANISM(S): Nicotiana benthamiana 
2018-10-24 | PXD006338 | Pride
Host-pathogen interactions Bead-free immunoprecipitation  plant-virus interactome  tandem mass spectrometry Luteoviridae Polerovirus Molecular virology Potato leafroll virus Phloem-limited 
ProteomicsComprehensive analysis of platelet glycoprotein Ibα glycosylation.
Background: Platelet glycoprotein (GP) Ibα is the major ligand-binding subunit of the GPIb-IX-V complex that binds von Willebrand Factor (VWF). GPIbα is heavily glycosylated, and its glycans have been proposed to play key roles in platelet clearance, VWF binding, and as target antigens in immune thr...
ORGANISM(S): Homo sapiens (Human) 
2023-02-09 | PXD035030 | Pride
Human Platelets Glycoproteins 
ProteomicsCell-type specific tagging of glycoproteins in murine-human co-culture samples
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected ce...
ORGANISM(S): Homo sapiens (Human) 
2022-09-10 | PXD035438 | Pride
Mcf7 Co-culture  lfq Proteomics Human-murine 4t1 Secretome 
ProteomicsA metzincin-like O-glycopeptidase from Akkermansia muciniphila requires the Tn antigen for cleavage of the peptide bond.
HDX-MS analysis of the protein ALT51, comparing deuterium uptake between the full length protein and a truncated construct missing the CBM-like domain. Mass spectrometry was used to map the cleavage sites of CAT in a selection of representitive mucin-like glycoproteins.
ORGANISM(S): Homo sapiens (Human) 
2022-08-31 | PXD034904 | Pride
Hdx-ms Glycoproteins Ms Alt51 
UnknownSerological responses to the first four doses of SARS-CoV-2 vaccine in patients with inflammatory bowel disease.
Not available
S-EPMC9597523 | biostudies-literature
UnknownLarge-scale mutational analysis identifies UNC93B1 variants that drive TLR-mediated autoimmunity in mice and humans.
Not available
S-EPMC11116816 | biostudies-literature
UnknownAdding 6 months of androgen deprivation therapy to postoperative radiotherapy for prostate cancer: a comparison of short-course versus no androgen deprivation therapy in the RADICALS-HD randomised controlled trial.
Not available
S-EPMC7616360 | biostudies-literature
UnknownWild canids and felids differ in their reliance on reused travel routeways.
Not available
S-EPMC12519222 | biostudies-literature
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