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ProteomicsProteomics analysis of physiological effect of ZBTB16 (PLZF) on osteogenic differentiation of human valve interstitial cells (VICs) by DDA PASEF on TimsToF Pro
During fundamental proteotranscriptomic analysis of human valve interstitial cells (VICs) osteogenic differentiation, we revealed ZBTB16 (PLZF) as one of the most upregulated proteins. Thus, to investigate its physiological role in VICs osteogenic differentiation we performed its overexpression by l...
ORGANISM(S): Homo sapiens (Human) 
2022-10-21 | PXD031572 | Pride
Zbtb16  plzf  osteogenic differentiation  valve interstitial cells  pasef  timstof pro 
ProteomicsComparative analysis of DDA- and DIA-PASEF methods for proteomics analysis of human valve interstitial cells and osteoblasts during osteogenic differentiation
Shotgun proteomics is one of the key "omics" methods, the methodology of which is rapidly developing. Mass spectrometers of the TimsToF series (Bruker) with ion mobility are one of the young technological platforms for shotgun proteomics in which both data dependent (DDA) and data independent acquis...
ORGANISM(S): Homo sapiens (Human) 
2024-11-12 | PXD036566 | Pride
Data independent acquisition Pasef Shotgun proteomics Homo sapiens Timstof pro Calcific aortic valve disease 
ProteomicsMolecular mechanisms of endothelial osteoinductive/osteosupressive properties
In vivo, osteogenesis is tightly connected with capillaries and endothelium assumed to be one of the main regulators of osteogenic differentiation of underling mesenchymal cell. In our experiments in vitro, we cultured human osteoblasts and human umbilical vein cord endothelial cells (HUVEC) in oste...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD037385 | Pride
Osteoblasts Timstof pro Osteogenic differeniation Endothelium Huvec 
ProteomicsProteomics comparison of human valve interstitial cells from healthy patients and donors with CAVD
Calcific aortic valve disease (CAVD) is an slowly progressive calcification of heart valve which leads to aortic stenosis. The only existing treatment of CAVD is surgical replacment of calcified valve - development of anti-CAVD treatment is urgent task. For better understanding of molecular mechanis...
ORGANISM(S): Homo sapiens (Human) 
2022-10-21 | PXD032212 | Pride
Osteogenic differentiation Q exactive Valve interstitial cells Cavd 
ProteomicsProteomics analysis of early stages of human osteoblasts osteogenic differentiation in vitro
Cellular mechanisms and signaling cascades underlying the early stages of osteoblast progenitors differentiation in adult bone are still not well understood. Therefore, we performed shotgun proteomics analysis of primary culture of isolated human osteoblasts from femur of adult donors in control and...
ORGANISM(S): Homo sapiens (Human) 
2022-12-25 | PXD033697 | Pride
Osteogenic differentiation Pasef Shotgun proteomics Timstof pro Osteoblasts Dda 
ProteomicsCrenigacestat inhibits osteogenic differentiation of human valve interstitial cells and seems promising for treatment of cardiovascular calcification
Vascular calcification is common pathology various forms of which presented in the half of humans. Calcific aortic valve disease (CAVD) is one of the most dangerous forms of vascular calcification. Despite high mortality there is no target therapy against CAVD. Thus, we tested crenigacestat (LY30394...
ORGANISM(S): Homo sapiens (Human) 
2022-10-04 | PXD029632 | Pride
Valve intersticial cells Timstof pro Vascular calcification Ly3039478 Notch inhibitors Crenigacestat Calcific aortic valve disease Shotgun proteomics 
ProteomicsComparative analysis of proteomes and secretomes of human mesenchymal stem cells isolated from six sources during osteogenic differentiation
Ability to perform osteogenic differentiation is one of the minimal criteria of mesenchymal stem cells (MSCs). Still, it is generally unknown whether osteogenic differentiation is universal cell fate or various phenotypically similar cell states. Besides this, MSCs and their secretomes are actively ...
ORGANISM(S): Homo sapiens (Human) 
2023-11-05 | PXD037320 | Pride
Msc Timstof pro Osteogenic differeniation Secretome 
ProteomicsAnalysis of HUVEC secretome and histone N-terminal acetylation after Notch activation
Vasculature permeate our entire body and involved in homeostasis and progression of various disease. Endothelium is a backbone of cardiovascular system and endothelial disfunction is associated with most forms of cardiovascular disease from atherosclerosis to chronic heart failure. Decrease in shear...
ORGANISM(S): Homo sapiens (Human) 
2023-02-15 | PXD032978 | Pride
Lc-msms Histone Notch pathway N-terminal acetylation Endothelium Timstof Huvec 
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