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Chloroplast group IIA introns derive from bacterial ribozymes. Their splicing likely requires Maturase K (MatK), which has been largely inaccessible to functional analyses being itself a chloroplast intron-encoded protein. Here we used co-immunoprecipitation coupled with proteomics to identify prote...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2026-02-10 | PXD060055 | Pride
Aconitum carmichaeli chloroplast matK gene for maturase K, Vietnam
Maturase K (MatK) is the only splicing factor encoded in the chloroplast genomes of land plants. Here we used a transplastomic Nicotiana tabacum line, in which the plastidial MatK gene is C-terminally tagged with a 3x-HA tag, to conduct size-exclusion chromatography of soluble protein extracts enric...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2026-02-10 | PXD069946 | Pride
Arabidopsis thaliana (Arabidopsis) MATURASE K INTERACTING PROTEIN1 (MKIP1) is a plastid-localized, non-canonical member of the starch-branching enzyme family. Here we used co-immunoprecipitation (IP) coupled with proteomics to identify proteins bound to MKIP1 in stably transformed Arabidopsis lines ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-02-10 | PXD060108 | Pride
Arabidopsis thaliana (Arabidopsis) MATURASE K INTERACTING PROTEIN1 (MKIP1) is a plastid-localized, non-canonical member of the starch-branching enzyme family. Inducible silencing of MKIP1 by estradiol-treatment results in pale newly emerged leaves. Here we analysed the proteome of these leaves from ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-02-10 | PXD067382 | Pride
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