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MetabolomicsMetabolome and proteome changes in skeletal muscle and blood of pre-weaning calves fed leucine and threonine supplemented diets

In pre-weaning calves, both leucine and threonine play important roles in growth and muscle metabolism. In this study, metabolomics, proteomics and clinical chemistry were used to assess the effects of leucine and threonine supplementation added to milk replacer on 14 newborn Holstein male calves...

2020-02-20 | MTBLS1045 | MetaboLights
ProteomicsProfiling antibody responses to M. tuberculosis proteins in TB suspects’ sera using full-proteome microarrays.
Understanding the immune response to tuberculosis requires greater knowledge of humoral responses. To characterize antibody targets and the effect of disease parameters on target recognition, we developed a systems immunology approach that integrated detection of antibodies against the entire Mycob...
ORGANISM(S): Homo sapiens 
2010-07-13 | E-GEOD-19433 | biostudies-arrayexpress
ProteomicsGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-08-02 | MSV000080014 | MassIVE
ProteomicsMetal organic layers enabled cell surface engineering coupling biomembrane fusion for dynamic membrane proteome profiling
Systematically dissecting the highly dynamic and tightly communicating membrane proteome of living cells is essential for systems-level understanding of fundamental cellular processes and intricate relationship between membrane-bound organelles constructed through membrane traffic. While extensive e...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD044945 | Pride
Membrane protein enrichment metal organic layers proteome 
ProteomicsProfiling proteome-scale antibody responses to M. tuberculosis
This SuperSeries is composed of the following subset Series: GSE30721: Profiling proteome-scale antibody responses to M. tuberculosis proteins in sera of macaques infected with M. tuberculosis GSE30722: Profiling proteome-scale antibody responses to M. tuberculosis proteins in TB suspect's sera Refe...
ORGANISM(S): Homo sapiens 
2012-07-05 | E-GEOD-31368 | biostudies-arrayexpress
ProteomicsMembrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamics
We combine the membrane mimetic (MM) Peptidisc with thermal proteome profiling (TPP) to screen membrane proteomes in detergent-free environments. Using whole mouse liver, we demonstrate the stabilization of integral membrane proteins (IMPs), specifically ATP-binding cassette transporters, through in...
ORGANISM(S): Mus musculus (Mouse) Escherichia coli 
2025-11-17 | PXD055093 | Pride
Membrane mimetic Mouse liver Thermal proteome profiling 
ProteomicsMembrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamic interactions
Integral membrane proteins (IMPs) are key targets for small-molecule therapeutics. However, robust, unbiased, and detergent-free methods to probe on- and off-target interactions within this protein class remain underdeveloped. Previously, we introduced the Peptidisc membrane mimetic (MM) for water-s...
ORGANISM(S): Mus musculus (Mouse) 
2025-11-17 | PXD068828 | Pride
Membrane proteins Orbitrap exploris Mus musculus Membrane mimetic thermal proteome profiling Proteomics 
ProteomicsDeep membrane proteome profiling of high-risk human paraganglioma
We combined glycopeptide enrichment by N-glyco-FASP (Zielinska et al., 2010), SPEG (Tian et al., 2007) with a hydrophobic segment-oriented hpTC method (Vít et al 2016) and a standard “detergent and trypsin” approach into a tree-pronged “Pitchfork” strategy for the analysis of membrane proteome in hi...
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD029344 | Pride
Lc-msms Paraganglioma Membrane proteome Human Pitchfork strategy 
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showi...
ORGANISM(S): Homo Sapiens (human) 
2016-09-08 | PXD004904 | JPOST Repository
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