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Previous systems-based proteomic approaches have characterized alterations in protein co-expression networks of unfractionated asymptomatic (AsymAD) and symptomatic Alzheimer’s disease (AD) brains. However, it remains unclear how sample fractionation and sub-proteomic analysis influences the organi...
ORGANISM(S): Homo sapiens (Human) 
2019-09-02 | PXD014376 | Pride
The study aimed to find out quantitative changes at the protein level in the total membrane fraction enriched in synaptosomes and mitochondria upon the absence of the dopamine transporter in rat brain. Therefore, the brain of male rats (Wister Han) DAT-wildtype and DAT-knockout animals, were dissect...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-05-19 | PXD028157 | Pride
The purpose of the present study was to validate the enrichment of transporter proteins in plasma membrane fraction prepared from mouse liver and kidney with Membrane Protein Extraction Kit by means of beads homogenize for efficient subcellular fractionation. The crude and plasma membrane fractions ...
ORGANISM(S): Mus Musculus (mouse) 
Pollen germination and subsequent pollen tube elongation are essential for successful land plant reproduction. These processes are achieved through well-documented activation of membrane trafficking and cell metabolism. Despite this, our knowledge of the dynamics of cellular phospholipids remains sc...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2022-10-27 | PXD037046 | Pride
Trypanosoma cruzi invades non-professional phagocytic cells by subverting their membrane repair process, which is dependent on membrane injury and cell signaling, intracellular calcium increase and lysosome recruitment. Cells lacking Lysosome Associated Membrane Proteins 1 and 2 are less permissive ...
ORGANISM(S): Trypanosoma cruzi 
2022-02-17 | PXD028897 | Pride
Rapid nerve conduction in the CNS is facilitated by the insulation of axons with myelin, a specialized oligodendroglial compartment distant from the cell body. Myelin is turned over and adapted throughout life; however, the molecular and cellular basis of myelin dynamics is not well understood. Hypo...
ORGANISM(S): Mus musculus 
Comparison of the CD34+CD38- versus CD34+CD38+ fractions of human umbilical cord blood and comparison of the slow-dividing fraction versus the fast dividing fraction of the CD34+/CD38- population.
ORGANISM(S): Homo sapiens 
Total protein and membrane-enriched fractions of Synechocystis sp. PCC 6803 wild type (WT) and mutant strains affected with inactivated DNA methyltransferase genes were analyzed by LC-HDMSE. The mutation of the DNA methyltransferase gene sll0729 encoding M.Ssp6803II initially led to a strong phenoty...
ORGANISM(S): Synechocystis sp. (strain PCC 6803 / Kazusa) 
2019-06-26 | PXD012698 | Pride
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