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ProteomicsQuantitative Measurement of the Requirement of Diverse Protein Degradation Pathways in MHC Class I Peptide Presentation
The adaptive immune system distinguishes self from non-self by surveying peptides generated from degradation of intracellular proteins that are loaded onto MHC Class I molecules for display on the cell surface. While early studies reported that the bulk of cell surface MHC Class I complexes require ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-02-15 | MSV000091285 | MassIVE
ProteomicsHLA-DO Modulates the Diversity of the MHC-II Self-peptidome
HLA-DR1 bound peptides of five biological replicates of LG2-WT(012916_WT1,022216_WT1, 02080316MHCIIWT1, 080316MHCIIWT2, 080316MHCIIWT3) and DO-KO Clone 1 LG-2 cells (012916_KO1, 022216_DO1, 081216MHCIIKO1, 080416MHCIIKO2, 080416MHCIIKO3) were processed in parallel. Each biological replicate has 3 te...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-07-03 | MSV000082570 | MassIVE
ProteomicsThe effect of interferons on presentation of defective ribosome products as HLA peptides
The class I major histocompatibility complex (MHC) bound peptides, produced from immature proteins that are degraded rapidly are called defective ribosome products (DRiPs). Such DRiPs are possibly involved with early alerting of the immune system about impeding infections, thus bringing about faster...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-10-25 | MSV000096209 | MassIVE
TranscriptomicsMHC Class I Peptides Convey Intracellular mTOR Signals to the Cell Surface
EL4 cells were treated or not with 20nM rapamycin for 48h. Total RNA was extracted from rapamycin-treated and untreated EL4 cells with TRIzol RNA reagent (Invitrogen) as instructed by the manufacturer. Samples were purified using DNase (Qiagen, Mississauga, ON, Canada) and the RNeasy Mini kit (Qiage...
ORGANISM(S): Mus musculus 
2010-12-01 | E-MEXP-3007 | biostudies-arrayexpress
ProteomicsIdentification of post-translationally modified MHC class 1-associated peptides
Over the past three decades, the Hunt laboratory has developed advancements in mass spectrometry-based technologies to enable the identification of peptides bound to major histocompatibility complex (MHC) molecules. The MHC class I processing pathway is responsible for presenting these peptides to c...
ORGANISM(S): Homo sapiens (Human) 
2025-04-15 | PXD061414 | Pride
Imac Post-translational modification Cancer immunotherapy Immunopeptidomics Mhc associated peptides 
ProteomicsMild acid elution and MHC immunoaffinity chromatography reveal similar albeit not identical profiles of the HLA immunopeptidome
To understand and treat immunology-related diseases, a comprehensive, unbiased characterization of major histocompatibility complex (MHC) peptide ligands is of key importance. Preceding the analysis by mass spectrometry, MHC peptide ligands are typically isolated by MHC immunoaffinity chromatography...
ORGANISM(S): Homo sapiens (Human) 
2020-11-11 | PXD012771 | Pride
Mhc immunoaffinity chromatography Cid Post-translational modification Hla ligandome Mild acid elution Cysteinylation Mhc bound peptides 
ProteomicsThermostability profiling of MHC-bound peptides: a new dimension in immunopeptidomics and design of immunotherapeutics.
The features of peptide antigens that contribute to their immunogenicity are poorly understood. Although the stability of peptide-MHC (pMHC) is known to be important, current assays assess this interaction only for peptides in isolation and not in the context of natural antigen processing and presen...
ORGANISM(S): Homo sapiens (Human) 
2020-11-30 | PXD017824 | Pride
Mhc Thermostability Immunopeptidome 
ProteomicsDiscovery of Non-Canonical Peptides Derived from Novel Small Open Reading Frames as MHC-I Epitopes
Immunopeptides that are translated in cells and presented at the cell surface by major histocompatibility complex (MHC) mole-cules are important epitopes in basic Immunology and translational cancer immunotherapy. While most of the reported immuno-peptides are derived from protein coding sequence, t...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD024415 | Pride
Price Ribotish Sorf Non-canonical peptides Lc-ms Mhc-i 
ProteomicsThermostability profiling of MHC-bound peptides: a new dimension in immunopeptidomics and design of immunotherapeutics
The features of peptide antigens that contribute to their immunogenicity are poorly understood. Although the stability of peptide-MHC (pMHC) is known to be important, current assays assess this interaction only for peptides in isolation and not in the context of natural antigen processing and presen...
ORGANISM(S): Homo sapiens (Human) 
2020-11-30 | PXD017839 | Pride
Mhc Thermostability Immunopeptidome 
ProteomicsDigging deeper into the HLA peptidome – Characterization of the post-translationally modified peptides
Peptides displayed by MHC molecules on a cell’s surface, referred to as its immunopeptidome, play an important role in the adaptive the immune response. Antigen processing for MHC class I presentation is a ubiquitous pathway present in all nucleated cells which generate and present peptides of both ...
ORGANISM(S): Homo sapiens (Human) 
2021-06-07 | PXD024562 | Pride
Spliced peptides Immunopeptidome Post-translational modifications Mhc class i Mhc binding motifs 
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