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ProteomicsUse of a modified Multiplexed Inhibitor Bead assay to study kinome responses to cytostatic treatment in human cancer cell lines Triplicate Test: pilot study
In order to improve elution of proteins from the column, to reduce the cost as well as the hands on time of the analysis, we here present a modification of the Multiplexed Inhibitor Bead assay (MIB assay), where we elute the proteins from beads containing covalently bound inhibitors using trypsin di...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080744 | MassIVE
TranscriptomicsRNA from 3 d and 2 d old embryos of mibta52b, mibm132 and mibtfi91, and RNA from 3 d, 2d and 1 d old embryos of mibta52b
The Notch signaling is an evolutionarily conserved signal transduction cascade that plays essential roles in a variety of developmental processes. In this study, we examined the mib mutants for defects in pancreas development using in situ hybridization and GFP expression analysis of pancreas-specif...
ORGANISM(S): Danio rerio 
2010-06-25 | E-GEOD-8522 | biostudies-arrayexpress
ProteomicsProteomic characterization of MDA-MB-231 and MCF-10A breast cells exposed to 1 mT 50 Hz ELF for 4 hours
MDA-MB-231 breast cancer cells and MCF-10A breast cells were exposed to 1 mT 50 Hz extremely low-frequency magnetic field (ELF-MF) for 4 hours
ORGANISM(S): Homo Sapiens (human) 
2023-02-17 | PXD040120 | JPOST Repository
ProteomicsUse of a modified Multiplexed Inhibitor Bead assay to study kinome responses to cytostatic treatment in human cancer cell lines
In order to improve elution of proteins from the column, to reduce the cost as well as the hands on time of the analysis, we here present a modification of the Multiplexed Inhibitor Bead assay (MIB assay), where we elute the proteins from beads containing covalently bound inhibitors using trypsin di...
ORGANISM(S): Hordeum vulgare (Barley) 
2017-02-13 | PXD005286 | Pride
Kinome Mib 
ProteomicsDetermining MELK inhibitor specificity using MIB/MS
The key objectives of this study were to evaluate the selectivity profiles of three MELK inhibitors, 8a, HTH, and OTS, using a cell-based assay, in order to identify a highly selective inhibitor to subsequently investigate MELK function. To this end, we utilized a chemical proteomics approach called...
ORGANISM(S): Homo sapiens (Human) 
2020-01-09 | PXD016022 | Pride
Chemoproteomics Lfq Kinase enrichment 
ProteomicsLabel-free proteomics to study the effect of IL-9 on MDA-MB-231 and HeLa cells
To study the proteomic profile of HeLa and MDA-MB-231 upon IL-9 stimulation, we treated both the cell-lines with the cytokine for 24 hours. Quantitative label-free proteomic analysis was then performed and abundance ratios were obtained for proteins from cells in IL-9 treated v. untreated conditions...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-03-03 | MSV000086990 | MassIVE
Proteomicsdezocine pull down LC-MSMS in MDA-MB-231 CELLS
To reveal the direct target and molecular mechanisms underlying the effects of dezocine, a pull-down assay and HPLC-MS/MS were performed.l the direct target and molecular mechanisms underlying the effects of dezocine, a pull-down assay and HPLC-MS/MS were performed.
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD022583 | Pride
Breast cancer Lc-msms Human Mda-mb-231 
ProteomicsProteome of Control versus shKDM2B in MDA-MB-231 Cells
Project comparing the proteome between control and knockdown of KDM2B in MDA-MB-231 cell lines. Previous data demonstrated that knockdown of KDM2B induced transcriptional changes in ribosomal and metabolic gene targets and thus TMT proteomics was pursued to compare the protein level changes that occ...
ORGANISM(S): Homo sapiens (Human) 
2023-11-22 | PXD046970 | Pride
Human Breast cancer Tmt proteomics Mda-mb-231 Kdm2b 
ProteomicsAPIM-peptide targeting PCNA improves cisplatin therapy in a rat muscle-invasive bladder cancer model
High mortality rate of muscle-invasive bladder cancer (MIBC) and complexity of disease demand new multi-targeting treatment strategies. Targeting proliferating cell nuclear antigen (PCNA) with a peptide containing the AlkB homologue 2 PCNA interacting motif (APIM), is shown to impair multiple vital ...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-09-10 | PXD008724 | Pride
Ay-27 Rat Mib Lfq Assay 
ProteomicsMDA-MB-231 Breast Cancer Bone Tropic vs Parental Hypoxic Secretome Analysis
The project profiled the expression patterns in hypoxia induced secretomes between MDA-MB-231 parental and MDA-MB-231 Bone Tropic (BT) breast cancer cell lines which have been previously generated by Massague and colleagues (Kang et al. Cancer Cell 2003).
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080797 | MassIVE
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