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ProteomicsComparision of Nano and Micro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD024169 | Pride
Single shot proteomics Nano-flow lc Micro-flow lc 
ProteomicsMicro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Homo sapiens (Human) 
2021-07-01 | PXD023587 | Pride
Single shot proteomics Micro-flow lc 1.0 mm id column 
Proteomicsmicro-proteomics experiment for single meiotic-phase barley anthers
We describe here a new micro-proteomics workflow for the analysis of individual barley anthers. The workflow detects more than 4000 proteins from small amounts of material representing single or paired anther samples, covering a dynamic range of protein expression levels across five orders of magnit...
ORGANISM(S): Hordeum vulgare (Barley) 
2019-05-04 | PXD010887 | Pride
Barley Micro-proteomics Anther 
ProteomicsMASP micro-specimen samples at different spatial locations
Quantitative protein mapping on whole-tissue levels provides important insights into the spatially-organized regulatory processes/networks related to diseases and therapy, but remains a tremendous challenge. We describe a micro-scaffold assisted spatial proteomics(MASP) method, based on precise tiss...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-11 | PXD037041 | Pride
Masp Lc-msms 
UnknownMicro-proteomics with iterative data analysis: Proteome analysis in C. elegans at the single worm level.
Not available
S-EPMC4819713 | biostudies-literature
UnknownCombination of automated sample preparation and micro-flow LC-MS for high-throughput plasma proteomics.
Not available
S-EPMC9824974 | biostudies-literature
ProteomicsImproved Sensitivity in Low-Input Proteomics using Micro-Pillar Array-based Chromatography
Capitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nano-LC-ESI-MS/MS is currently the most sensitive analytical technology for the comprehensive characterization of complex protein samples. However, despite tremendous technological impr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014124 | Pride
Nano-lc-esi-ms/ms nano-flow hplc hela cell digest 
ProteomicsMicro-proteomics with iterative data analysis: proteome analysis in C. elegans at the single worm level
Proteomics studies typically analyze proteins at a population level, using extracts prepared from tens of thousands to millions of cells. The resulting measurements correspond to average values across the cell population and can mask considerable variation in protein expression and function between ...
ORGANISM(S): Caenorhabditis elegans 
2015-11-17 | PXD002948 | Pride
Eukaryote C.elegans 
ProteomicsEfficient micro-scale basic reverse phase peptide fractionation for global and targeted proteomics
This work describes a micro-scale basic reverse phase liquid chromatographic (bRPLC) fractionation method that offers high reproducibility and efficiency for peptide mixtures from small (5-20 g) samples. Shotgun and targeted proteomic analysis were performed to detect differentially expressed prote...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-02-09 | MSV000079500 | MassIVE
MetabolomicsConnecting structure and function from organisms to molecules in small-animal symbioses through chemo-histo-tomography
Our understanding of metabolic interactions between small symbiotic animals and bacteria or parasitic eukaryotes that reside within their bodies is extremely limited. This gap in knowledge originates from a methodological challenge, namely to connect histological changes in host tissues induced by b...
2021-07-06 | MTBLS2639 | MetaboLights
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