Sort   by:  
 Page size 
ProteomicsSuspension TRAPping filter (sTRAP) sample preparation for quantitative proteomics in the low µg input range using a plasmid DNA micro-spin column: analysis of the hippocampus from the 5xFAD Alzheimer’s Disease mouse model
Suspension TRAPping filter (sTRAP) is an attractive sample preparation method for proteomics study. Here, we demonstrated the use of a low-cost Plasmid DNA spin column for sTRAP. We first evaluated, the reproducibility of this protocol and the low CoV using 4 replicates of 5-10 µg of a synapse enric...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-23 | PXD041045 | Pride
Alzheimer’s disease Proteomics 5xfad mouse model Micro-spin column Strap 
UnknownSuspension TRAPping Filter (sTRAP) Sample Preparation for Quantitative Proteomics in the Low µg Input Range Using a Plasmid DNA Micro-Spin Column: Analysis of the Hippocampus from the 5xFAD Alzheimer's Disease Mouse Model.
Not available
S-EPMC10177283 | biostudies-literature
TranscriptomicsTranscription profiling of Theiler stage 17 wild type mouse anterior vs posterior mouse sclerotome
This experiment was performed in order to identify transcriptional differences between the anterior- and posterior-halves of mouse sclerotome. Cells-derived from the anterior- and posterior-sclerotome-halves from maturing mouse somites were compared to identify transcripts that are differentially ex...
ORGANISM(S): Mus musculus 
2006-11-16 | E-MEXP-913 | biostudies-arrayexpress
TranscriptomicsRNA sequencing on FACS-isolated satellite cells 7 days after injury in Tibialis Anterior Muscles
The aim of this project is to study satellite cells functionality and get an unprecedented insight into their transcriptional landscape during muscle injury healing process. We aim at revealing their gene expression profiles and describe the continuous variations in their transcriptome after the inv...
ORGANISM(S): Mus musculus 
2026-08-21 | GSE303612 | GEO
TranscriptomicsIntegrative Molecular Concepts Modeling of Prostate Cancer Progression
Using laser capture microdissection to isolate over 100 specific cell populations, we report the profiling of prostate cancer progression from benign epithelium to metastatic disease. By analyzing these expression signatures in the context of over 15,000 â??molecular conceptsâ??, or sets of biologic...
ORGANISM(S): Homo sapiens 
2010-06-05 | E-GEOD-6099 | biostudies-arrayexpress
ProteomicsTh17 and MAIT17 cells are associated with severe Recessive Dystrophic Epidermolysis Bullosa: Staphylococcus aureus involvement
Recessive Dystrophic Epidermolysis bullosa (RDEB) is characterized by wounds colonized with Staphylococcus aureus associated with local and systemic inflammation. However, it remains unclear how the immune response and S. aureus interact. A novel proteomic pipeline was used to evaluate over 800 prot...
ORGANISM(S): Homo sapiens (Human) 
2025-07-21 | PXD042141 | Pride
Severe recessive dystrophic epidermolysis bullosa Dia Plasma Staphylococcus aureus Th17 and mait17 cells 
TranscriptomicsTranscriptomic analysis of chlorhexidine shock in Acinetobacter baumannii
In this study the transcriptomic changes occurring in A. baumannii ATCC 17978 in response to shock treament using the biocide chlorhexdine were determined. A. baumannii ATCC 17978 cells were grown at 37°C with shaking (200rpm) in 35mL cultures in MH broth to OD600=0.75, at which time they were spli...
ORGANISM(S): Acinetobacter baumannii 
2013-12-03 | E-GEOD-51525 | biostudies-arrayexpress
TranscriptomicsPoly(A) tail length profiling of mRNAs from fission yeast
To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarr...
ORGANISM(S): Schizosaccharomyces pombe 
2007-05-16 | E-TABM-217 | biostudies-arrayexpress
ProteomicsMapping Myc Interactome by Micro-Affinity Purification Coupling Mass Spectrometry
Affinity purification-mass spectrometry (AP-MS) is a powerful approach for protein-protein interaction (PPI) analysis. Here, we report the development of a micro-scale immobilized metal affinity chromatography (m-IMAC) platform for rapid and reproducible purification of protein complexes prior to ma...
ORGANISM(S): Homo sapiens (Human) 
2026-06-09 | PXD073401 | Pride
Transcriptional regulation mechanisms Proteomics Affinity purification-mass spectrometry C-myc 
ProteomicsInteractome of CFTR using Apex and TurboID proximity labelling
The proteins interacting with CFTR and its mutants have been intensively studied using different experimental approaches, enabling to better understand the cellular processes leading to proper protein folding, routing to the plasma membrane, recycling, activation and degradation. Recently, several s...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD035184 | Pride
Apex Turboid Kcnk3 Cftr Interactome 
Sort   by:  
 Page size