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MetabolomicsProteomic analysis of osteocyte line (MLO-Y4) with knockout of cystic fibrosis transmembrane conductance regulator (CFTR) or chloride deprivation treatment

Mass spectrometry–based proteomic analysis of an osteocyte line, MLO-Y4, was conducted to profile global protein expression changes under two experimental settings, 1) CRISPR-Cas9-based knockout of CFTR versus wild-type; 2) Cl- deprivation versus normal extracellular medium.

2026-03-27 | MTBLS14165 | MetaboLights
TranscriptomicsGlobal gene expression profile of small intestine from wild-type and CFTR-knockout mice.
The objective of this study was to identify changes in gene expression levels between wild-type and CFTR-knockout small intestine. CFTR-knockout mice (provided by Dr. Lane Clarke of the University of Missouri) were maintained on colyte. Keywords: gene expression comparison Four wild-type and four C...
ORGANISM(S): Mus musculus 
2010-05-25 | E-GEOD-8763 | biostudies-arrayexpress
ProteomicsCFTR interactome
The uploaded files are the RAW files used to generate the CFTR interactome dataset. The raw data were searched with ProLuCID against the human IPI database version 3.23 and search results were filtered with DTASelect 2.1.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-08-13 | MSV000079235 | MassIVE
GenomicsDifferential contribution of cis-regulatory elements to higher order chromatin structure and expression of the CFTR locus
Higher order chromatin structure establishes domains that organize the genome and coordinate gene expression. However, the molecular mechanisms controlling transcription of individual loci within a topological domain (TAD) are not fully understood. The cystic fibrosis transmembrane conductance reg...
ORGANISM(S): Homo sapiens 
2016-01-07 | E-GEOD-74709 | biostudies-arrayexpress
TranscriptomicsA microRNA network regulates expression and biosynthesis of CFTR and CFTR-M-NM-^TF508.
Production of functional proteins requires multiple steps including gene transcription and post-translational processing. MicroRNAs (miRNA) can regulate individual stages of these processes. Despite the importance of the cystic fibrosis transmembrane conductance regulator (CFTR) channel for epithe...
ORGANISM(S): Homo sapiens 
2012-09-04 | E-GEOD-38956 | biostudies-arrayexpress
TranscriptomicsCFTR dependent response
Wild-type and dF508 CFTR mutant human bronchial epithelial cells were uninfected or infected for 3 hours at an MOI of 30-50 with P.aeruginosa strain PAO1. Each cell line was tested in 4 replicates. Each replicate consists of 4 data sets: 2 sets of duplicate spots on 2 arrays. The two arrays in each...
ORGANISM(S): Homo sapiens 
2010-06-09 | E-GEOD-2357 | biostudies-arrayexpress
TranscriptomicsRNA-seq of HUVECs in two models of CFTR blockade
To unravel CFTR function in endothelial cells (HUVECs), we used two complementary approaches to induce CFTR blockade: (i) the allosteric CFTR inhibitor CFTRinh-(172) which is commonly used in CF research, and (ii) CFTR-specific short hairpin RNAs (shRNA).
ORGANISM(S): Homo sapiens 
2020-10-31 | E-MTAB-8599 | biostudies-arrayexpress
ProteomicsElexacaftor/VX-445-mediated CFTR interactome remodeling reveals differential correction driven by mutation-specific translational dynamics
Cystic fibrosis (CF) is one of the most prevalent lethal genetic diseases with over 2000 identified genetic variants in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Pharmacological chaperones such as Lumacaftor (VX-809), Tezacaftor (VX-661) and Elexacaftor (VX-445) treat muta...
ORGANISM(S): Homo sapiens (Human) 
2023-09-18 | PXD039773 | Pride
Ap-ms Elexacaftor Cf Cftr Trikafta Interactomics 
TranscriptomicsExpression data from transfected IB3 cells transfected with CFTR mutants
CFTR plasmid constructs were transfected in CF Bronchial IB3 cell cultures using Lipofectamine 2000™. Global gene mRNA expression profiles at 48 hr after transfection were created with the Affymetrix U133 chip set. We used microarrays to detail the global changes in gene expression occuring as a re...
ORGANISM(S): Homo sapiens 
2011-09-30 | E-GEOD-28072 | biostudies-arrayexpress
ProteomicsSecretome and Proteome of WT and CFTR-KO pancreatic duct epithelial cells
Loss of CFTR function in the pancreatic duct leads to dysregulated luminal pH causing premature activation of digestive enzymes and tissue necrosis. Drastic alterations in pancreatic tissue architecture and cellular composition changes the microenvironment of the islets. Given that CFTR is expressed...
ORGANISM(S): Mustela putorius furo (European domestic ferret) (Mustela furo) 
2021-05-27 | PXD025126 | Pride
Pancreas Cystic fibrosis Cystic fibrosis related diabetes Ductal cells Proteome Secretome 
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