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MetabolomicsEvaluation of sample preparation methods for NMR-based metabolomics of cow milk
The quality of milk metabolome analyzed by nuclear magnetic resonance (NMR) is greatly influenced by the way samples are prepared. Although this analytical method is increasingly used to study milk metabolites, a thorough examination of available sample preparation protocols for milk has not been re...
2019-06-13 | MTBLS623 | MetaboLights
MetabolomicsMetabolome and proteome changes in skeletal muscle and blood of pre-weaning calves fed leucine and threonine supplemented diets

In pre-weaning calves, both leucine and threonine play important roles in growth and muscle metabolism. In this study, metabolomics, proteomics and clinical chemistry were used to assess the effects of leucine and threonine supplementation added to milk replacer on 14 newborn Holstein male calves...

2020-02-20 | MTBLS1045 | MetaboLights
ProteomicsWhole milk proteome of human and rhesus macaque during peak lactation
Milk has been well established as the optimal nutrition source for infants, yet there is still much to be understood about its molecular composition. Therefore, our objective was to develop and compare comprehensive milk proteomes for human and rhesus macaques to highlight differences in neonatal nu...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Macaca Mulatta (ncbitaxon:9544) 
2015-02-23 | MSV000079045 | MassIVE
ProteomicsHuman milk dynamics: Inter-individual variation in milk proteome
The aim of this study was to map inter-individual variation in the human milk proteome and to investigate relationships between these data and possible explanations for observed variation.
ORGANISM(S): Homo sapiens (Human) 
2022-05-04 | PXD028280 | Pride
Homo sapiens  breast milk  proteomics  proteins  variability 
Proteomicsmilk-derived extracellular vesicles UHPLC-MS
Breast milk contains macromolecular components with distinctive functions, whereby milk fat globules and casein micelles provide nutrition, while whey contains molecules that stimulate the infant’s immunity. Interestingly, the importance and function of extracellular vesicles (EV) in milk has not be...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD004423 | Pride
Lc-ms Human milk Vesicles Milk proteome 
ProteomicsAntibody-independent identification of bovine milk-derived peptides in breast-milk
Exclusively breast-fed infants can exhibit clear signs of IgE or non IgE-mediated cow’s milk allergy. The definite characterization of dietary cow’s milk proteins (CMP) that survive the maternal digestive tract to be absorbed into the bloodstream and secreted into breast milk remains missing. The ai...
ORGANISM(S): Homo sapiens (Human) 
2016-07-19 | PXD003657 | Pride
Bovine Peptides Human Milk Lc-ms/ms 
ProteomicsPersonalized Human Milk Profiling
Human milk is the truest form of personalized nutrition, supporting dynamic needs of the infant with important nutritional and bioactive constituents that change throughout lactation. Additionally, human milk is individual specific and is unique for each mother-infant dyad. Proteins and endogenous p...
ORGANISM(S): Homo sapiens (Human) 
2021-02-09 | PXD014917 | Pride
Human milk Immunity Endogenous peptides Personalized nutrition Proteins 
ProteomicsInfluence of Lactococcus cremoris and iodophor-based teat disinfectants on the cow milk proteome
In a recent study, a teat disinfectant based on a nisin-producing Lactococcus cremoris displayed comparable efficacy to a conventional iodophor disinfectant in preventing dairy cow mastitis when implemented in the dairy cow milking routine. Here, we carried out a longitudinal study for an in-depth i...
ORGANISM(S): Bos taurus (Bovine) 
2024-10-17 | PXD045030 | Pride
Proteomics  nanolc-ms/ms 
ProteomicsPeptidomics analysis of milk protein-derived peptides released over time in the preterm infant stomach
Over the course of milk digestion, native milk proteases and infant digestive proteases fragment intact proteins into peptides with potential bioactivity. This study investigated the release of peptides over three hours of gastric digestion in 14 preterm infant sample sets. The peptide content was e...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2019-03-12 | PXD012192 | Pride
Gastric Human milk Preterm Digestion Peptide Bioactive 
ProteomicsAllergen Shedding in Human Milk from Mothers of Preterm Infants: Proteomic and Peptidomic Feasibility and Pilot Analysis
There is little information regarding the allergen content of milk feeds in the preterm population. Previous studies have evaluated specific proteins/peptides via ELISA, but no studies have performed a broad analysis of the allergenic peptide content and protease activity of milk feeds in this popul...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2024-01-26 | PXD033473 | Pride
Human Allergen Native paptides Milk 
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