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MetabolomicsThe Metabolic Fate of Oxaliplatin in the Biological Milieu Investigated During In Vivo Lung Perfusion Using a Unique Miniaturized Sampling Approach Based on Solid-Phase Microextraction Coupled with Liquid Chromatography-Mass Spectrometry

Adjuvant chemotherapy after pulmonary metastasectomy for colorectal cancer may reduce recurrence and improve survival rates; however, the benefits of this treatment are limited by the significant side effects that accompany it. The development of a novel in vivo lung perf...

2022-08-02 | MTBLS4982 | MetaboLights
ProteomicsMiniaturized sample preparation on a digital microfluidics device for sensitive bottom-up microproteomics of mammalian cells using magnetic beads and mass spectrometry-compatible surfactants
We used digital microfluidics (DMF) for the sample preparation of approximately 100 mammalian cells, and subsequent bottom-up proteome analysis by LC-MS. This comprised optimization of cell lysis conditions for DMF, the development of detergent-buffer systems, and adaptation of the single-pot, solid...
ORGANISM(S): Homo sapiens (Human) 
2019-10-07 | PXD014158 | Pride
Digital microfluidics Detergents Sp3 Microproteomics Miniaturization 
ProteomicsFilter aided, single tip (FAST) method for high throughput, ultrasensitive proteomics sample preparation reveals proteome asymmetry in Xenopus embryos
We report a filter aided, single tip (FAST) method for ultrasensitive proteomic sample preparation. To minimize sample loss and contamination, the method reduces the surface area of the filter to ~0.1 mm2, the total volume of reagents to < 10 μL, and the number of sample transfer steps to two. 25,88...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2022-07-12 | PXD015175 | Pride
Single blastomere Sample preparation Ultrasensitive proteomics 
ProteomicsDe novo Classification of Mouse B Cell Types using Surfaceome Proteotype Maps
System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when sample is limiting. We miniaturized and automated the previously described Cell Surface Capture technology increasing sensitivity, reproducibility, and throughput. We...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-12-11 | PXD013627 | Pride
Flow cytometry Cell surface capture Cell surface proteome Hrm B-cell development Dda Mouse model Classification Affinity purification Dia Lc-ms Proteotype Autocsc Csc Surfaceome 
ProteomicsNovel integrated trace sample preparation unraveled the proteome transformation during mesenchymal-amoeboid transition
This is a novel sample preparation for proteomic analysis of rare cells
ORGANISM(S): Homo Sapiens Mus Musculus 
2021-07-03 | PXD027085 |
UnknownMiniaturized Filter-Aided Sample Preparation (MICRO-FASP) Method for High Throughput, Ultrasensitive Proteomics Sample Preparation Reveals Proteome Asymmetry in Xenopus laevis Embryos.
Not available
S-EPMC7931810 | biostudies-literature
ProteomicsAn Optimized Miniaturized Filter Aided Sample Preparation Method for Sensitive Cross-linking Mass Spectrometry Analysis of Microscale Samples
Cross-linking mass spectrometry (XL-MS) is a powerful tool for elucidating protein structures and protein-protein interactions (PPIs) at the global scale. However, sensitive XL-MS analysis of mass-limited samples remains challenging, due to serious sample loss during sample preparation of the low-ab...
ORGANISM(S): Homo sapiens (Human) 
2025-01-03 | PXD051322 | Pride
Cross-linking mass spectrometry Microscale samples 
UnknownA miniaturized spectrometer for NMR relaxometry under extreme conditions.
Not available
S-EPMC6673705 | biostudies-literature
UnknownAutomation of RNA-Seq Sample Preparation and Miniaturized Parallel Bioreactors Enable High-Throughput Differential Gene Expression Studies.
Not available
S-EPMC12029635 | biostudies-literature
UnknownLight-sheet microscopy enabled by a miniaturized plane illuminator.
Not available
S-EPMC11729294 | biostudies-literature
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