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ProteomicsSimple Strategy of Integrating the Micro-centrifugation-based Extraction and 4-Hexylphenylazosulfonate for the Preparation of Plant Microsomal Proteins for Bottom-Up Proteomics
The preparation of high-quality microsomal proteins (MPs) is of critical importance to high-throughput microsomal proteomics. The isolation of MPs for proteomic analysis has to date relied on Ultracentrifugation-based methods (UBMs), however, these methods are labor-intensive, time-consuming, and un...
ORGANISM(S): Oryza sativa subsp. japonica (Rice) 
2021-09-29 | PXD025132 | Pride
Rice leaves Perseus Label-free quantitative proteomics Ultracentrifugation Micro-centrifugation Azo Maxquant Microsomal membrane extraction Plasma membrane Sds 
TranscriptomicsDegradome analysis in Phalaenopsis aphrodite subsp. formosana
We performed one degradome sequencing for identification and characterization of novel microRNAs in Phalaenopsis aphrodite subsp. formosana. Plant tissues of leaves, stalk, and flower buds frozen in liquid nitrogen were ground to fine powder using a mortar and pestle, respectively. Total RNA extract...
ORGANISM(S): Phalaenopsis aphrodite subsp. formosana 
2012-11-06 | E-GEOD-35739 | biostudies-arrayexpress
UnknownA Self-Powered Double U-Finger MME Resonator Capable of Wirelessly Capturing Abnormal Message in Smart Grid Networks.
Not available
S-EPMC12463033 | biostudies-literature
ProteomicsArginine and lysine methylproteomes of Giardia duodenalis
Methylation is a common post-translational modification of lysine and arginine in eukaryotic proteins. To date, these methylomes are best characterised in model organisms and higher eukaryotes. Herein, we integrated bioinformatics, proteomics and high-content drug-screening to comprehensively explor...
ORGANISM(S): Giardia lamblia ATCC 50803 
2020-08-07 | PXD016813 | Pride
Methylation Arginine methylation Ptm Giardia Lysine methylation 
TranscriptomicsLongSAGE analysis of wild-type mouse brain regions
LongSAGE libraries generated from various brain regions of 8- to 12-week-old male C57BL/6 mice. Keywords: Micro-dissection, Brain regions, SAGE Sau3A I was used as the anchoring enzyme and Mme I as the tagging enzyme. Data are provided after linker removal.
ORGANISM(S): Mus musculus 
2008-02-05 | E-GEOD-7593 | biostudies-arrayexpress
UnknownQuantitative analysis of single particle trajectories: mean maximal excursion method.
Not available
S-EPMC2849086 | biostudies-literature
UnknownA high-throughput method for cloning and sequencing human immunodeficiency virus type 1 integration sites.
Not available
S-EPMC1642152 | biostudies-literature
ProteomicsTMT-based quantitative membrane proteomics reveals in-depth investigation of PRRs involved in the perception of MSP1 in rice leaves
Pathogen secreted pathogen-associated molecular patterns (PAMPs) play a key role in PAMPs triggered immunity (PTI) in plant for the recognition of pathogens. In rice, fewer PAMPs and their pattern recognition receptors (PRRs) have been characterized during rice-Magnaporthe oryzae interaction. Partic...
ORGANISM(S): Oryza sativa (Rice) 
2022-08-12 | PXD032689 | Pride
Msp1 Tmt Plant-pathogen interaction Prrs Magnaporthe oryzae Mme method Rice 
TranscriptomicsCpG site methylation of HCV-cirrhotic, HCV-HCC, and normal liver tissues
In this study we used a high-throughput method for assaying methylation of CpG sites simultaneously in a single sample for identifying differences in methylation observed in tissues ranging from normal liver to pre-neoplastic (cirrhosis) and neoplastic (HCC) states. Since there are important clinica...
ORGANISM(S): Homo sapiens 
2010-02-18 | E-GEOD-18081 | biostudies-arrayexpress
GenomicsDNA accessibility profile of three C. elegans tissues during development
We show there is minimal genome-wide chromatin rearrangements (as measured by DNA accessibility) during tissue differentiation in C. elegans transgenic expression of E. coli DAM (DNA Adenine Methyltransferase) from tissue-specific promoters followed by mapping of methylated sites using a procedure t...
ORGANISM(S): Caenorhabditis elegans 
2011-04-16 | E-GEOD-23042 | biostudies-arrayexpress
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