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TranscriptomicsTranscription profiling of SrcR388A/Y527F or SrcD386N/Y527F stably transfected SYF cells serum-starved overnight and then treated with imidazole 5 mM for 1 h or 12 reveals that the protein tyrosine kinase Src R388A mutant can be rescued in live cells using the small molecule imidazole
SrcR388A/Y527F or SrcD386N/Y527F stably transfected SYF cells were serum-starved overnight and then treated with imidazole 5 mM for 1 h or 12 h. RNeasy Miniprep column (Qiagen) was used to prepare total RNA from each treatment group. All samples were run in commercial arrays from Affymetrix,
ORGANISM(S): Mus musculus 
2007-12-12 | E-GEOD-4043 | biostudies-arrayexpress
ProteomicsExercise intensity modulates the human plasma secretome and interorgan communication (proteomics part)
Exercise is recognized as first-line therapy for many cardiometabolic diseases, including obesity, type 2 diabetes, and hypertension. Despite the abundant health-promoting effects of exercise, in-depth characterization of circulatory factors that mediate these benefits in humans remains incomplete. ...
ORGANISM(S): Homo sapiens (Human) 
2026-06-25 | PXD069170 | Pride
Physiology Metabolism Exercise 
ProteomicsThe resting and ligand-bound states of the membrane-embedded human T-cell receptor–CD3 complex
The T-cell receptor (TCR) initiates T-lymphocyte activation, but the mechanism of TCR activation remains uncertain. Here, we present cryogenic electron microscopy structures for the unliganded and human leukocyte antigen (HLA)-bound human TCR–CD3 complex in nanodiscs that provide a native-like lipid...
ORGANISM(S): Homo sapiens (Human) 
2025-12-17 | PXD070648 | Pride
Tcr2 Disulfide bridge Membrane 
ProteomicsLRG1 is an adipokine that promotes insulin sensitivity and suppresses inflammation
While dysregulation of adipocyte endocrine function plays a central role in obesity and its complications, the vast majority of adipokines remain uncharacterized. We employed bio-orthogonal non-canonical amino acid tagging (BONCAT) and mass spectrometry to comprehensively characterize the secretome ...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-21 | PXD035318 | Pride
Lrg1 Adipokine Inflammation Insulin 
ProteomicsThe origin and evolution of the interactions between sex peptide and sex peptide receptor
Post-mating responses play a vital role in successful reproduction across diverse species. In fruit flies, sex peptide (SP) binds to sex peptide receptor (SPR), triggering a series of post-mating responses. However, the origin of SPR predates the emergence of SP. The evolutionary origins of the inte...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2024-03-20 | PXD044633 | Pride
Post-mating responses Sex peptide (sp) In fruit flies Sex peptide receptor (spr) 
ProteomicsMapping phosphorylations on C-terminus of receptor
Characterization of interaction of a GPCR complex with beta-arrestin. The phosphorylation landscape of the receptor β2V2R's C-terminal is studied with LC-MS.
ORGANISM(S): Spodoptera frugiperda 
2020-05-27 | PXD015298 | Pride
Phosphorylation In-gel digestion Arrestin Gpcr complex 
ProteomicsOptimization of ultracentrifugation-based method to enhance the purity and proteomic profiling depth of plasma-derived extracellular vesicles and particles
Circulating extracellular vesicles and particles (EVPs) are being investigated as potential biomarkers for early cancer detection, prognosis, and disease monitoring. However, the suboptimal purity of EVPs isolated from peripheral blood plasma has posed a challenge of in-depth analysis of the EVP pro...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD052726 | Pride
Extracellular vesicles and particles (evps)  early cancer detection  biomarkers  proteomics 
ProteomicsNon-reversible tissue fixation by EDC carbodiimide allows for imaging extracellular vesicles in situ
Extracellular vesicles (EVs) are secreted nanosized particles with many biological functions and a broad range of pathological associations. A major technical limitation to understanding the role of EVs in normal and diseased specimens has been the inability to visualize the spatial localization ...
ORGANISM(S): Bos taurus (Bovine) 
2019-11-18 | PXD015234 | Pride
Imaging extracellular vesicles fixation tissue 
ProteomicsCdc42 phosphorylation study - Cdc42 Regulates Neuronal Polarity during Cerebellar Axon Formation and Glial-Guided Migration
Govek et al. demonstrate conditional loss of Cdc42 in cerebellar granule cell progenitors (GCPs) perturbs GCP polarity and impairs axon patterning, glial-guided migration, and cerebellar foliation. Phospho-proteomic analysis identified polarity and cytoskeletal proteins as affected targets in Cdc42 ...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-22 | PXD008148 | Pride
Brain Mouse Phospho 
ProteomicsThe lipid droplet protein DHRS3 is a regulator of melanoma cell state
Lipid droplets are fat storage organelles composed of a protein envelope which surrounds a lipid rich core. Dynamic regulation of this protein envelope underlies differential lipid droplet formation and function in diverse cell types. In melanoma, the ability to form lipid droplets has been linked t...
ORGANISM(S): Homo sapiens (Human) 
2025-11-10 | PXD050693 | Pride
Lipid droplet Dhrs3 Melanoma Human 
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