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ProteomicsSTUDY OF THE PLASMA MEMBRANE PROTEOME DYNAMICS REVEALS NOVEL TARGETS OF THE NITROGEN REGULATION IN YEAST
Yeast cells, to be able to grow on a wide variety of nitrogen sources, regulate the set of nitrogen transporters present at their plasma membrane. Such regulation relies on both transcriptional and post-translational events. While large-scale microarray studies have identified most nitrogen-sensitiv...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-07-10 | PXD005273 | Pride
Yeast plasma membrane Proteomics Transporters Nitrogen sources Endocytosis Arrestin 
Proteomicsα-arrestin-dependent endocytosis after cycloheximide treatment
To identify new plasma membrane (PM) proteins that require α-arrestins for endocytosis, we performed an iTRAQ-based quantitative proteomic screening. We analysed and compared the PM proteome for each arrestin knockout (art∆) strain to a WT strain after a 90 min cycloheximide (CHX) treatment. CHX is ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-09-25 | PXD014695 | Pride
Endocytosis Arrestin Plasma membrane Cycloheximide 
ProteomicsQuantitative proteomics on HeLa cell surface (iTraq)
While several clathrin-independent endocytic processes have been described so far, their biological relevance often remains elusive, especially in pathophysiological contexts such as cancer. In this study, we find that the tumor marker CD166/ALCAM (Activated Leukocyte Cell Adhesion Molecule) is a cl...
ORGANISM(S): Homo sapiens (Human) 
2020-04-22 | PXD017526 | Pride
Hela Itraq 
ProteomicsLactate racemase proteins MALDI TOF/TOF
This project reports the characterization of the lactate racemase system in Lactobacillus plantarum. The lactate racemase (LarA) is a nickel-dependent enzyme. The apo-enzyme (apoLarA) is activated by a single nickel-containing maturation protein (LarE) that requires preactivation by two other access...
ORGANISM(S): Lactobacillus plantarum 
2014-04-02 | PXD000775 | Pride
Lactate racemase Lactobacillus plantarum Nickel enzyme Maturation proteins 
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