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ProteomicsNpl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA
RNA-binding proteins (RBPs) control every RNA metabolic process by multiple protein-RNA and protein-protein interactions. Their roles have largely been analyzed by crude mutations, which abrogate several functions at once and likely impact the structural integrity of the large mRNP assemblies, these...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD035153 | Pride
Rna-binding sites Npl3 Sr-like protein Mrnp assembly Structure Xl-ms Rna-binding proteins Nmr Mrna Gene expression 
TranscriptomicsTranscriptome maps of mRNP biogenesis factors define pre-mRNA recognition
Biogenesis of eukaryotic messenger ribonucleoprotein complexes (mRNPs) involves the synthesis, splicing, and 3M-bM-^@M-^Y-processing of pre-mRNA, and the assembly of mature mRNPs for nuclear export. We mapped 23 mRNP biogenesis factors onto the newly synthesized yeast transcriptome, providing ~10^5-...
ORGANISM(S): Saccharomyces cerevisiae 
2014-07-23 | E-GEOD-59676 | biostudies-arrayexpress
TranscriptomicsA time-resolved framework for the recruitment of mRNP processing and assembly factors to a site of transcription
Processing and packaging of messenger ribonucleoprotein (mRNP) particles involve complex, coordinated interactions between nascent transcripts, RNA binding proteins (RBPs), and other associated factors. Despite the critical role of co-transcriptional mRNP assembly in gene expression, the temporal dy...
ORGANISM(S): Saccharomyces cerevisiae 
2025-07-24 | GSE293820 | GEO
ProteomicsNpl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA
Assessing the function of the RNA-binding activity of RNA-binding proteins (RBPs) is challenging. Here, Keil et al. use a targeted XL-ms approach to identify RNA-binding sites in components of nuclear mRNPs in S. cerevisiae. The analysis of RNA-binding mutants of one selected example, the SR-like pr...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD034656 | Pride
Affinity purification Rna-processing Lfq Npl3 Spliceosome 
UnknownNpl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA.
Not available
S-EPMC9881175 | biostudies-literature
UnknownA time-resolved framework for the recruitment of mRNP processing and assembly factors to a site of transcription.
Not available
S-EPMC12359037 | biostudies-literature
UnknownStructural basis for high-order complex of SARNP and DDX39B to facilitate mRNP assembly.
Not available
S-EPMC10508174 | biostudies-literature
UnknownThe Survival of Motor Neuron Protein Acts as a Molecular Chaperone for mRNP Assembly.
Not available
S-EPMC5492976 | biostudies-literature
UnknownHighly Overexpressed AtC3H18 Impairs Microgametogenesis via Promoting the Continuous Assembly of mRNP Granules.
Not available
S-EPMC9335048 | biostudies-literature
GenomicsA time-resolved framework for the recruitment of mRNP processing and assembly factors to a site of transcription
A time-resolved framework for the recruitment of mRNP processing and assembly factors to a site of transcription
PRJNA1246517 | ENA
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