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ProteomicsFilamin C domain 18-21 in vitro kinase assay
In vitro kinase assay with human filamin C domain 18-21 recombinantly expressed in E.coli, purified by Ni2+-NTA beads and incubated with PKC alpha, Akt or a combination of both to identify kinase specific phosphorylation sites.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-25 | PXD008893 | Pride
Filamin c Akt In vitro kinase assay Pkb Pkcalpha 
ProteomicsFunctional proteomics identifies acinus L as a direct insulin- and amino acid-dependent mTORC1 substrate
The serine/threonine kinase mammalian target of rapamycin (mTOR) governs growth, metabolism, and aging in response to insulin and amino acids (aa), and is often activated in metabolic disorders and cancer. Much is known about the regulatory signaling network around mTOR, but surprisingly few direct ...
ORGANISM(S): Homo sapiens (Human) 
2015-04-28 | PXD001245 | Pride
Lc-ms Human Ms-based in vitro kinase assay Mtor 
ProteomicsFenbendazole Met ID_in vitro_cross-species_FBMN
Fenbendazole Met ID by an integrative approach using feature-based molecular networking of cross-species in vitro liver microsomal and hepatocellular incubation.
ORGANISM(S): Canis Lupus Familiaris (ncbitaxon:9615) Macaca Fascicularis (ncbitaxon:9541) Cd1 Mouse Homo Sapiens (ncbitaxon:9606) 
2024-08-08 | MSV000095554 | MassIVE
ProteomicsALPK3 Whole Cell Lysate In vitro Kinase assay and phosphoproteomics
A whole cell lysate in vitro kinase assay using ALPK3 was performed to identify novel substrates.
ORGANISM(S): Homo sapiens (Human) 
2023-01-19 | PXD038544 | Pride
Alpk3 
ProteomicsIdentification of phosphorylation sites of transcription factor after reaction with protein kinase
In previous study, we reported that MdERF17 contributes to Chl degradation in apple fruit peels. Here, we demonstrated that MdERF17 and MdMPK4 interact with each other in vitro and in vivo and raised the possibility that MdMPK4 may contribute to fruit degreening via post-transcriptional regulation o...
ORGANISM(S): Malus domestica 
2022-05-20 | PXD030037 | Pride
Phosphorylation，in vitro kinase assay，lc-ms/ms 
ProteomicsAntagonist actions of CMK-1/CaMKI and TAX-6/Calcineurin along the C. elegans thermal avoidance circuit orchestrate nociceptive habituation
In this project, we performed three sets of in vitro kinase assays coupled to phosphoproteomic workflows. (1) tryptic peptides of C. elegans lysate treated with recombinant active CMK-1 (positive2 run) and kinase-dead CMK-1 and 18O labeled ATP (2) immobilized whole cell lysate (native conditions, p...
ORGANISM(S): Caenorhabditis elegans 
2025-04-10 | PXD055776 | Pride
Lc-msms In vitro kinase assay Kinase Phosphorylation 
ProteomicsSILAKin: A novel high throughput SILAC-based quantitative mass spectrometry assay to identify the substratome of kinases secreted by pathogens
We developed SILAkin, a novel and easy method to identify substrates that is applicable to most kinases. It combines phosphatase treatment, pulse heating, in vitro kinase assay and SILAC (Stable Isotope Labeling with Amino acids in Cell culture)-based quantitative mass spectrometry (MS). SILAkin was...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026220 | Pride
Casein kinase 1 Host-pathogen interactions Substrate screen Leishmania 
ProteomicsD6 PROTEIN KINASE plasma membrane polarity is dependent on a repeated CXX(X)P motif and serine phosphorylation
D6 PROTEIN KINASE (D6PK) is a polarly localized plasma membrane-associated kinase from Arabidopsis thaliana that activates polarly distributed PIN-FORMED (PIN) auxin transporters. D6PK traffics rapidly to and from the plasma membrane, independently from its PIN targets. D6PK plasma membrane associat...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-23 | PXD037885 | Pride
Arabidopsis D6 protein kinase Phosphorylation Polarity 
ProteomicsIdentifying receptor kinase substrates using an 8,000 peptide kinase client library enriched for conserved phosphorylation sites
In eukaryotic organisms, protein kinases regulate diverse protein activities and signaling pathways through phosphorylation of specific protein substrates. Isolating and characterizing kinase substrates is vital for defining downstream signaling pathways. The Kinase Client (KiC) assay is an in vitro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-02-26 | PXD056072 | Pride
Candidates P2k1 Phosphopeptide 
ProteomicsIdentifications of protein kinase A substrates with quantitative phosphoproteomics and primary-sequence based scoring.(H-89)
Protein kinase A (PKA) is a serine/threonine kinase who plays essential roles in the complex signalling pathways. To better understand the functionalities, it is necessary to elucidate direct interplays between PKA and their substrates in living system. In order to screen them in a high-throughput m...
ORGANISM(S): Homo Sapiens (human) 
2018-02-15 | PXD005925 | JPOST Repository
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