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ProteomicsBoosting MS1-only proteomics with machine learning allows 2000 protein identifications in single-shot human proteome analyses using 5-minute HPLC gradients
Proteome-wide analyses rely on tandem mass spectrometry and extensive separation of proteolytic mixtures imposing considerable instrumental time consumption that is one of the main obstacles in a broader acceptance of proteomics in biomedical and clinical research. Recently, we presented a fast prot...
ORGANISM(S): Homo sapiens (Human) 
2021-03-18 | PXD023977 | Pride
Faims Fusion lumos Mass spectrometry Ms1-only Hela Protein identification 
ProteomicsA Rapid and Sensitive Single-cell Proteomic Method Based on Fast LC Separation, Deep-learning RT Prediction and MS1-only Acquisition
We report an integrate strategy that combines fast liquid chromatography (LC) separation, deep learning-based retention time (RT) prediction and MS1-only acquisition for sensitive and rapid single-cell proteome analysis. Finally, SCP-MS1 was applied to sorafenib resistant HepG2 cells.
ORGANISM(S): Homo Sapiens 
PXD036211 |
ProteomicsMS1-only data acquisition of human gut microbiome data treated with 5 drugs at different concentrations.
We tested the ability of MS1-only data to identify the effects of drugs at varying concentrations on a human gut microbiome. We processed a human stool sample and cultured the processed microbiome in vitro under anaerobic condition for 48 hours using the rapidAIM assay. The microbiome was treated wi...
ORGANISM(S): human gut metagenome 
2022-07-29 | PXD024815 | Pride
Ms1-only Human gut microbiome 
ProteomicsPotential of Negative Ion Mode Proteomics: MS1-Only Approach
Advancing Negative Ion Mode Proteomics. The main objective of the project is the exploration of the unconvetional negative ion mode for proteomics studies. In this work, we thoroughly studied the best chromatographic conditions for negative ion mode proteomics before testing different enzymatic dige...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD040583 | Pride
Ms/ms free Proteomics Negative ion mode 
ProteomicsDirectMS1Quant: ultrafast quantitative proteomics with MS/MS-free mass spectrometry. Part 1/2
Recently, we presented the DirectMS1 method of ultrafast proteome-wide analysis based on minute-long LC gradients and MS1-only mass spectra acquisition. Currently, the method provides the depth of human cell proteome coverage of 2500 proteins at 1% false discovery rate (FDR) when using 5-min LC grad...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2023-08-25 | PXD033350 | Pride
Dia E.coli Ups Ms1-only 
ProteomicsDirectMS1Quant: ultrafast quantitative proteomics with MS/MS-free mass spectrometry. Part 2/2
Recently, we presented the DirectMS1 method of ultrafast proteome-wide analysis based on minute-long LC gradients and MS1-only mass spectra acquisition. Currently, the method provides the depth of human cell proteome coverage of 2500 proteins at 1% false discovery rate (FDR) when using 5-min LC grad...
ORGANISM(S): Homo sapiens (Human) 
2023-08-25 | PXD033355 | Pride
Dda lfq Tmt Glioblastoma Ms1-only 
UnknownPotential of Negative-Ion-Mode Proteomics: An MS1-Only Approach.
Not available
S-EPMC10407931 | biostudies-literature
UnknownRIPPER: a framework for MS1 only metabolomics and proteomics label-free relative quantification.
Not available
S-EPMC4920113 | biostudies-literature
ProteomicsOn the utility of ultrafast MS1-only proteomics in drug target discovery studies based on thermal proteome profiling method
Advances in high-throughput high-resolution mass spectrometry and the development of thermal proteome profiling approach (TPP) have made it possible to accelerate a drug target search. Since its introduction in 2014, TPP quickly became a method of choice in chemical proteomics for identifying drug-t...
ORGANISM(S): Homo sapiens (Human) 
2024-10-07 | PXD051712 | Pride
Ultra-short gradient hplc-ms Thermal proteome profiling Drug targets Chemical proteomics 
ProteomicsIntegrated omics approaches to explore a new system of genetic control of dibenzothiophene desulfurization in Gordonia alkanivorans strain 135 -- MS1
This project aims to explore a new system for genetic control of dibenzothiophene desulfurization in Gordonia alkanivorans strain 135 using integrated omics approaches. A quantitative protomics, RNA-Seq analysis was performed. RNA-seq results were further validated using qRT-PCR for differentially e...
ORGANISM(S): Gordonia alkanivorans 
2025-05-07 | PXD058641 | Pride
Dibenzothiophene Ms1-only G. alkanivorans 135 Directms1 
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