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ProteomicsMSPathFinder publication datasets
These are datasets that accompany the MSPathFinder software publication. There are a set of 10 replicates of ovarian cancer tumor. These are prefixed with "CPTAC_intact_rep". There are also datasets for an A vs. B study with five replicates each. These are prefixed with "CPTAC_Intact_CR33" or "CPTAC...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-20 | MSV000080257 | MassIVE
UnknownA Markov Chain Monte Carlo Method for Estimating the Statistical Significance of Proteoform Identifications by Top-Down Mass Spectrometry.
Not available
S-EPMC6484843 | biostudies-literature
ProteomicsIsolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
This is the test data file for a protocol developed to effectively extract intact histones from sorghum leaf materials for profiling of histone post-translational modifications. Histones were extracted from isolated sorghum leaf nuclei and subjected to top down analysis without enzymatic digestion. ...
ORGANISM(S): Sorghum Bicolor (ncbitaxon:4558) 
2020-07-16 | MSV000085770 | MassIVE
ProteomicsTop-Down Mass Spectrometry Analysis of Histone Modifications in Sorghum during Drought Acclimation
This study explores the changes in histone modifications of sorghum bicolor through developmental stages and in response to drought stress in two sorghum genotypes. We analyzed the leaves of 48 plants using top-down mass spectrometry and identified 26 unique histone proteins and 677 unique histone p...
ORGANISM(S): Sorghum bicolor 
2019-10-30 | PXD014660 | Pride
Post-translational modifications Mass spectrometry Top-down Plant Epigenetics Histone clipping 
ProteomicsInvestigation of phycobilisome degradation using top down proteomics in Synechococcus elongatus UTEX 2973
Top down mass spectrometry analysis of Synechococcus elongatus UTEX2973 cyanobacteria under nitrogen starvation over time course of 4 hours. A number of phycobiliproteins covalently linked to phycocyanobilin pigments were detected as intact forms. Some of them showed partial degradation of the prote...
ORGANISM(S): Synechococcus elongatus 
2019-08-27 | PXD014590 | Pride
Phycobilisomes Light harvesting complexes Nitrogen starvation Photosynthesis Top down proteomics Protein degradation 
ProteomicsTop-Down proteomics and Bottom-Up terminomics for the Detection of Protein Termini in C. elegans
Here we investigate how top-down proteomics (TDP) can be of value for the detection of protein termini. Either GELFrEE or solid-phase enrichment was conducted for pre-fraction. To verify the detected N-termini HUNTER (High-efficiency Undecanal-based N Termini EnRichment) was employed. Reductive dime...
ORGANISM(S): Caenorhabditis elegans 
2022-09-26 | PXD033668 | Pride
Orbitrap q exactive plus Gelfree Mspathfinder Top-down proteomic C. elegans Orbitrap fusion lumos Informed proteomics Bottom-up proteomics Prosight 
ProteomicsUltrasensitive Top-Down Proteomics Analysis of Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform
We developed an ultrasensitive top down platform by incorporating a microfluidic sample preparation system, termed nanoPOTS (Nanodroplet Processing in One pot for Trace Samples), into a top down workflow. We determined that a unique combination of a nonionic detergent DDM with urea as a protein extr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-22 | MSV000084612 | MassIVE
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