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ProteomicsTop-Down proteomics and Bottom-Up terminomics for the Detection of Protein Termini in C. elegans
Here we investigate how top-down proteomics (TDP) can be of value for the detection of protein termini. Either GELFrEE or solid-phase enrichment was conducted for pre-fraction. To verify the detected N-termini HUNTER (High-efficiency Undecanal-based N Termini EnRichment) was employed. Reductive dime...
ORGANISM(S): Caenorhabditis elegans 
2022-09-26 | PXD033668 | Pride
Orbitrap q exactive plus Gelfree Mspathfinder Top-down proteomic C. elegans Orbitrap fusion lumos Informed proteomics Bottom-up proteomics Prosight 
ProteomicsThiol reactive tandem mass tags for quantitative top-down proteomics
Here, we describe the development of a labeling strategy for TDP targeting thiol groups of cysteine residues with iodoTMTsixplex. While this method inherently excludes the quantification of cysteine-free proteoforms, it provides the opportunity of sixplexing and the implementation of multidimensiona...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2021-08-06 | PXD026310 | Pride
Isobaric labeling Gelfree High ph fractionation Yeast Iodotmtsixplex Quantitative top-down proteomics Saccharomyces cerevisae Escherichia coli 
ProteomicsDenaturing and native top-down venomics: Mapping proteoforms and protein complexes from king cobra venom
Characterizing whole proteins by top-down proteomics avoids a step of inference encountered in the dominant bottom-up methodology when peptides are assembled computationally into proteins for identification. The direct interrogation of whole proteins and protein complexes from the venom of Ophiophag...
ORGANISM(S): Ophiophagus hannah (King cobra) (Naja hannah) 
2016-05-18 | PXD003403 | Pride
King cobra venom Top-down proteomics Proteoforms Native mass spectrometry Protein complexes 
ProteomicsHDL Separation with CN-GELFrEE and Intact Apolipoprotein Proteoform Analysis
Intact mass analysis in high resolution for CN-GELFrEE fractions of HLD particles
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-03-29 | MSV000091584 | MassIVE
ProteomicsCombination of bottom up 2D-LC-MS and intact protein separation enhances coverage of proteome and low molecular weight short open reading frame encoded peptides of the archeon Methanosarcina mazei
The recent discovery of an increasing number of small open reading frame (sORF) creates the need for suitable analytical technologies for the comprehensive identification of the corresponding gene products. In the present study we evaluated analytical approaches which will allow for simultaneous ana...
ORGANISM(S): Methanosarcina mazei Go1 
2016-09-26 | PXD004325 | Pride
Gelfree High ph lc High ph 2d-lc Top-down Methanosarcina mazei 
UnknownIn-depth proteomic analysis of mouse cochlear sensory epithelium by mass spectrometry.
Not available
S-EPMC3777728 | biostudies-literature
ProteomicsAn integrated landscape of mRNA and protein isoforms
Cellular processes like alternative splicing (AS) and proteolytic processing generate various protein isoforms from the same protein-coding gene. Studies using high-throughput sequencing indicate that about 90% of multi-exon genes in humans undergo AS. These AS events often occur in a tissue and dev...
ORGANISM(S): Homo sapiens (Human) 
2026-07-07 | PXD062269 | Pride
Pacbio Isoforms Proteogenomics Sds-gel Protein processing Tmt Alternative splicing 
UnknownOnline matrix removal platform for coupling gel-based separations to whole protein electrospray ionization mass spectrometry.
Not available
S-EPMC4419745 | biostudies-literature
UnknownPreparative Electrophoresis for HDL Particle Size Separation and Intact-Mass Apolipoprotein Proteoform Analysis.
Not available
S-EPMC10436667 | biostudies-literature
UnknownMapping Proteoforms and Protein Complexes From King Cobra Venom Using Both Denaturing and Native Top-down Proteomics.
Not available
S-EPMC4937514 | biostudies-literature
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