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ProteomicsEvaluation of N8-propargyl spermidine and spermine as substrates of deoxyhypusine synthase in the in vitro hypusination of eIF5A and RIPK1
eIF5A is a well-established protein substrate of deoxyhypusine synthase (DHS), which also requires spermidine as a small-molecule substrate. At the end of 2024, a publication claimed that RIPK1 is an additional DHS substrate and that N8-propargyl spermidine can also serve as a DHS substrate. To eval...
ORGANISM(S): Homo sapiens (Human) 
2026-03-13 | PXD071577 | Pride
N8-propargyl spermidine Dhs Eif5a Chemical probe Hypusination Deoxyhypusine synthase Ripk1 
ProteomicsEvaluation of N8-propargyl spermidine as a substrate of deoxyhypusine synthase in cellulo
Two complementary experiments were performed to evaluate whether N8-propargyl spermidine is a substrate of deoxyhypusine synthase in cells. HEK293 and mouse embryonic fibroblast (MEF) cells were incubated with either N8-propargyl spermidine or spermidine, lysed, and subjected to CuAAC ligation with ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2026-03-13 | PXD071477 | Pride
Chemical probe Hypusination Hek293 N8-propargyl spermidine Mef 
ProteomicsN8-propargyl spermidine as a chemical probe for sensing of ALDH1A1 activity in cells
A549 cells were incubated with N8-propargyl spermidine, N1-propargyl spermidine, or native spermidine. Protein conjugates formed in cellulo were analyzed as follows. After cell lysis, probe-protein adducts were subjected to bioorthogonal CuAAC ligation and affinity enrichment on NeutrAvidin beads. T...
ORGANISM(S): Homo sapiens (Human) 
2026-05-28 | PXD070993 | Pride
Chemical probe Aldh1a1 A549 Activity-based probe N8-propargyl spermidine 
ProteomicsEvaluation of N8-propargyl spermidine (Probe 1) as a substrate of deoxyhypusine synthase in cellulo
Two complementary experiments were performed to evaluate whether N8-propargyl spermidine is a substrate of deoxyhypusine synthase in cells. HEK293 and mouse embryonic fibroblast (MEF) cells were incubated with either N8-propargyl spermidine (Probe 1) or spermidine, lysed, and subjected to CuAAC liga...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2026-06-23 | PXD080059 | Pride
N8-propargyl spermidine Mef Chemical probe Hypusination Hek293 
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