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ProteomicsNative holdup (nHU) to measure binding affinities from cell extracts
Characterizing macromolecular interactions is essential for understanding cellular processes, yet nearly all methods used to detect protein interactions from cells are qualitative. Here, we introduce the native holdup (nHU) approach to quantify equilibrium binding constants and explore binding mecha...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD036024 | Pride
Mass spectrometry Protein-protein interaction Affinity constant determination 
ProteomicsNative holdup (nHU) to measure binding affinities from cell extracts
Characterizing macromolecular interactions is essential for understanding cellular processes, yet nearly all methods used to detect protein interactions from cells are qualitative. Here, we introduce a native holdup (nHU) approach that requires minimal sample preparation, investigates binding mechan...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD034790 | Pride
Protein interaction Mass spectrometry Native holdup 
ProteomicsInteractome of the oncogenic deltaNp73alpha isoform in human papillomavirus 38 E6/E7-transformed keratinocytes
DeltaNp73alpha is a major oncogenic isoform of tumor suppressor p73. We report deltaNp73alpha protein binding partners in the organelle and cytoplasmic compartments of a cellular model consisting of human keratinocytes transformed by the E6 and E7 oncoproteins of the beta-HPV38 virus, which is assoc...
ORGANISM(S): Homo sapiens (Human) 
2024-12-06 | PXD056377 | Pride
Human keratinocytes Tumor suppressor p73 Interactome 
ProteomicsIdentification and quantitative characterization of protein-DNA interactions with the native holdup assay: GATA1 regulation of the plasma membrane calcium pump
Polymorphisms in regulatory regions of the genome can alter RNA and protein expression and are implicated in a wide range of diseases. It has been recently discovered that a common haplotype in the erythroid-specific promoter of the ATP2B4 gene alters the expression of Plasma Membrane Calcium ATPase...
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD055181 | Pride
Protein-dna interaction Gata1 Native holdup 
ProteomicsThe E2F4/p130 repressor complex cooperates with oncogenic Np73 to promote cell survival
Tumor suppressor p53 and its related proteins, p63 and p73, can be synthesized as multiple isoforms lacking part of the N- or C-terminal regions. Specifically, high expression of the Np73 isoform is notoriously associated with poor prognosis in cancer. Here, we have performed proteomics analysis of ...
ORGANISM(S): Homo sapiens (Human) 
2023-02-28 | PXD022947 | Pride
Cellular transformation E2f4 np73 Gene expression Protein-protein interactions P53 
ProteomicsMetalloprotease-mediated cleavage of CD95 Ligand: QTOF Xevo XS data
CD95 is a member of the TNF receptor superfamily that is ubiquitously expressed in healthy and pathological tissues. Stimulation of CD95 by its physiological ligand CD95L induces its oligomerization leading in turn to the transduction of either apoptotic or non-apoptotic signals. CD95L can exist as ...
ORGANISM(S): Homo sapiens (Human) 
2023-02-20 | PXD039211 | Pride
Cd95l Metalloprotease Proteolytic cleavage 
ProteomicsTranscriptional repression activity of androgen receptor targets cell plasticity in prostate cancer: kinetic analysis after DHT treatment
Androgen receptor (AR) signaling remains the key therapeutic target in the management of hormone-naïve advanced prostate cancer (PCa) and castration-resistant PCa (CRPC). Recently, landmark molecular features have been reported for CRPC, including the expression of constitutively active AR variants ...
ORGANISM(S): Homo sapiens (Human) 
2022-10-13 | PXD029520 | Pride
Androgen receptor  ar-v7  cell plasticity  prostate cancer  emt  transcriptional repression 
ProteomicsTranscriptional repression activity of androgen receptor targets cell plasticity in prostate cancer: BioID analysis
Androgen receptor (AR) signaling remains the key therapeutic target in the management of hormone-naïve advanced prostate cancer (PCa) and castration-resistant PCa (CRPC). Recently, landmark molecular features have been reported for CRPC, including the expression of constitutively active AR variants ...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD029454 | Pride
Androgen receptor  ar-v7  cell plasticity  prostate cancer  emt  transcriptional repression 
ProteomicsA novel blood brain barrier-permeable IRE1 inhibitor prevents Glioblastoma relapse in mice
Inositol Requiring Enzyme 1 (IRE1) is a bifunctional serine/threonine kinase and endoribonuclease that is a major mediator of the Unfolded Protein Response (UPR) during endoplasmic reticulum (ER) stress. Tumor cells experience ER stress due to adverse environmental cues such as hypoxia or nutrient s...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD026908 | Pride
Ire1 Glioblastoma Unfolded protein response Inhibitors Endoplasmic reticulum 
ProteomicsReflux of Endoplasmic Reticulum proteins to the cytosol yields inactivation of tumor suppressors
In the past decades many studies reported the presence of Endoplasmic Reticulum (ER) resident proteins in the cytosol. However the mechanisms by which these proteins relocate and whether they exert cytosolic functions remain unknown. We found that select ER luminal proteins accumulate in the cytosol...
ORGANISM(S): Homo sapiens (Human) 
2021-05-03 | PXD023567 | Pride
Tumor cells Upr Endoplasmic reticulum 
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