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ProteomicsNHS esters are non-innocent protein acylating reagents
N-Hydroxysuccinimide (NHS)-ester derivatives represent one of the most widely used reagents in biological chemistry and chemical biology. Their perceived utility as acylating agents –intermediates that are sufficiently stable to be isolated (even in commercial form) yet active enough to allow direct...
ORGANISM(S): Escherichia coli 
2025-04-27 | PXD056955 | Pride
Ring-opening Nhs esters N-succinamide derivatives 
ProteomicsNHS esters are non-innocent protein acylating reagents
N-Hydroxysuccinimide (NHS)-ester derivatives represent one of the most widely used reagents in biological chemistry and chemical biology. Their perceived utility as acylating agents –intermediates that are sufficiently stable to be isolated (even in commercial form) yet active enough to allow direct...
ORGANISM(S): Escherichia coli Bacteria 
2025-04-27 | PXD056949 | Pride
Ring-opening reaction N-hydroxysuccinimide (nhs)-ester derivatives N-succinamide derivatives 
GenomicsSingle-cell RNA-seq analysis of syngeneic tumors treated with intratumoral NHS-rmIL12 immunotherapy
Single-cell RNA-seq analysis of syngeneic tumors treated with intratumoral NHS-rmIL12 immunotherapy
PRJNA774881 | ENA
UnknownAddressing NHS Chemistry: Efficient Quenching of Excess TMT Reagent and Reversing TMT Overlabeling in Proteomic Samples by Methylamine.
Not available
S-EPMC12004380 | biostudies-literature
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide (NHS) chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches. The labels are introduced onto primary Serines, tyrosines, and threon...
ORGANISM(S): Homo sapiens (Human) 
2025-03-14 | PXD054730 | Pride
Nhs Methylamine Hydroxylamine N-hydroxysuccinimide O-acylation 
UnknownConjugation of Native-Like HIV-1 Envelope Trimers onto Liposomes Using EDC/Sulfo-NHS Chemistry: Requirements and Limitations.
Not available
S-EPMC7589475 | biostudies-literature
ProteomicsNHS Carbamate Cross-Linkers Provide Complementary XL-MS Restraints via Preferential N-Terminal Targeting
Cross-linking mass spectrometry (XL-MS) has become a valuable tool for investigating the structural morphology and plasticity of proteins. Traditional cross-linkers contain two N-hydroxy succinimide (NHS) esters that mainly react with lysine residues. In this work, we optimized the in-solution react...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2026-03-30 | PXD069252 | Pride
Crosslinking ms Cross-linking ms Dsso carbamate Nhs carbamate cross-linkers N-terminal reactivity Xl-ms Cross-linking mass spectrometry Ms-cleavable cross-linkers 
ProteomicsIdentification of Lysine-Cysteine Crosslinking Sites in Homobifunctional NHS-Ester Crosslinkers
NHS esters are commonly used reactive groups in crosslinking chemistry. We first demonstrated that NHS esters can react with the side-chain thiol group of cysteine and observed lysine-cysteine (K-C) crosslinks when using homobifunctional crosslinkers. However, in conventional crosslinking workflows,...
ORGANISM(S): Homo Sapiens Bos Taurus 
2026-03-19 | PXD075905 |
ProteomicsClick-linking: a cell-compatible protein crosslinking method based on click chemistry
We developed a novel approach to in situ crosslinking mass spectrometry (XL-MS) where the reaction is split into two sequential and orthogonal coupling events. The method involves pre-stabilization of the proteome using a fixation protocol, followed by a two-step process that begins with extensive l...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-01-28 | PXD059118 | Pride
In situ Crosslinking Proteome-wide Protein-protein interactions Interactome 
ProteomicsUVPD of peptides modified with N-terminal UV-absorbing chromophores
Ultraviolet photodissociation (UVPD) has emerged as a powerful alternative to conventional collision-induced dissociation (CID) for peptide and protein sequencing in mass spectrometry–based proteomics. However, UVPD efficiency depends on the presence of UV-absorbing chromophores within analytes.The ...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD075227 | Pride
Chromophores Lc-ms Proteomics Nhs chemistry Uvpd 
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