Holm oak (Quercus ilex) is the most important and representative specie of the Mediterranean forest and of the Spanish agrosilvo-pastoral ecosystem “Dehesa”. Despite its environmental and economic interest, Holm oak is still an orphan species whose biology is very little known, especially at the mol...
Twenty microliter aliquots of BALF samples were dissolved in SDS-sample buffer and applied onto a 4-12% Nupage gel with MOPs running buffer. The run stopped after the samples migrated approximately ¼ distance into the gel. Each lane of the gel was sliced into smaller pieces, and subjected to destai...
Proteins were extracted from the cement glands of the cypird barnacle Megabalanus volcano. And thenproteins were subjected to in-solution digestion and analyzed by LTQ-Orbitrap Elite coupled to an Easy-nLC.
H157 cells transfected with vector and overexpressed AACT plasmids were treated and used for MS detection. The MS data were acquired using an Orbitrap Exploris 480 Mass Spectrometer coupled with an EASY-NLC 1200 liquid phase LC-MS system. DIA raw data files were analyzed by DIA-NN software. The diff...
During translation, transfer RNAs (tRNAs) deliver specific amino acids to the ribosome in a coordinated manner with the sequence encoded by the mRNA. Despite their central role in protein synthesis, the precise contribution of tRNAs to the modulation of translation remains poorly understood, prim...
Proteomic analysis: A stable isotope labeling approach (SILAC) was used to study changes in protein expression from murine macrophages in response to 3h of interaction with C. albicans (1:1 ratio) . Proteins were identified by Gel-LC-MS (1D SDS-PAGE, trypsin digestion and analysis by EASY-nLC LTQ O...
Endogenous peptides are extracted and seperated with nLC with HCD fragmentation on a LTQ Orbitrap Velos. Data analysis was done with Mascot and MaxQuant
Aim of the study was to profile and quantify the proteins head region of D. melanogaster that are changing as a result of phytohormone administration. Data-independent acquisition (DIA) bases quantitative proteomics approach were used to achieve this aim. An Orbitrap Fusion Tribrid mass spectrometer...
This dataset contains label-free immunoprecipitation mass spectrometry (IP-MS) data to identify interacting partners of target proteins in human cell lines. Two sample groups (OMM2.3-input, OMM2.3-IP) were generated via co-immunoprecipitation with anti-HBO1 antibody. All LC-MS/MS proteomic detection...
