Sort   by:  
 Page size 
UnknownA Structure-Based Mechanism for DNA Entry into the Cohesin Ring.
Not available
S-EPMC7507959 | biostudies-literature
ProteomicsCrosslinking/mass spectrometry analysis of Human Separase-Scc1-fusion and cohesin SMC1/SMC3 complexes
In this study, we employed the photoactivatable crosslinker (sulfo-SDA) to investigate protein-protein interaction between human Separase-Scc1 fusion protein and SMC1/SMC3 cohesin subunits complex in the presence of DNA.
ORGANISM(S): Homo sapiens (Human) 
2025-08-28 | PXD061667 | Pride
Sulfo-sda Smc1 Smc3 Scc1 Separase Xlms 
ProteomicsCryo-EM structures of dynactin’s shoulder and pointed end show their complex architecture and reveal conserved cargo adaptor binding sites
Dynactin is a 1.2 MDa complex that activates the molecular motor, dynein, for ultra-processive transport along microtubules. In order to do this it forms a tripartite complex with dynein and a coiled-coil adaptor. Dynactin consists of an actin-related filament whose length is defined by its flexible...
ORGANISM(S): Homo sapiens (Human) 
2021-03-19 | PXD020084 | Pride
Dynactin Crosslinking ms 
ProteomicsA folded conformation of MukBEF and Cohesin
PleasStructural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, where they have key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through topological entrapment and active translocation of DNA, but th...
ORGANISM(S): Escherichia coli 
2019-03-01 | PXD012370 | Pride
Condensin Structural maintenance of chromosomes Loop extrusion Smc proteins Crosslinking Mukbef Cohesin Dna translocation Kleisin 
ProteomicsA folded conformation of MukBEF and Cohesin
Structural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, where they have key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through topological entrapment and active translocation of DNA, but the und...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-03-01 | PXD012377 | Pride
Condensin Structural maintenance of chromosomes Loop extrusion Smc proteins Crosslinking Mukbef Cohesin Dna translocation Kleisin 
ProteomicsReprogramming the Lipid Peroxidation Product 4-ONE as a Chemoselective Cleavable Crosslinker
Nature builds and stabilizes proteins through covalent biological glues, yet the logic and traceability of metabolite-driven crosslinking remain obscure. Here we reprogram the lipid peroxidation product 4-oxo-nonenal (4-ONE) into a metabolite-derived Cys-Lys biological glue and mass-cleavable crossl...
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD077887 | Pride
4-one Peroxidation Mass spectrometry Crosslink Lipid 
ProteomicsReprogramming the Lipid Peroxidation Product 4-ONE as a Chemoselective Cleavable Crosslinker
Nature builds and stabilizes proteins through covalent biological glues, yet the logic and traceability of metabolite-driven crosslinking remain obscure. Here we reprogram the lipid peroxidation product 4-oxo-nonenal (4-ONE) into a metabolite-derived Cys-Lys biological glue and mass-cleavable crossl...
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD077820 | Pride
4-one Peroxidation Mass spectrometry Crosslink Lipid 
ProteomicsPurified Smc5/6 complex exhibits DNA substrate recognition and compaction
Investigation into the topology and fucntion of the yeast SMC5/6 complex
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-02-10 | PXD016196 | Pride
Crosslinking bs3 smc5/6 complex 
ProteomicsCryo-EM structures of holo condensin reveal a subunit flip-flop mechanism
This data is from BS3 crosslinked condensin tetramer How protein complexes of the SMC family fold DNA into the large loops that are fundamental for the 3D organization of genomes is a central unresolved question of chromosome biology. We used electron cryomicroscopy to investigate the reaction cycle...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-07-28 | PXD019275 | Pride
Condensin Tetramer Bs3 Crosslinking 
ProteomicsCryo-EM structures of holo condensin reveal a subunit flip-flop mechanism
This data is from sulfo-SDA crosslinked condensin pentamer. Two datsets, one without atp aand one with ATP. How protein complexes of the SMC family fold DNA into the large loops that are fundamental for the 3D organization of genomes is a central unresolved question of chromosome biology. We used el...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-07-28 | PXD019274 | Pride
Condensin Bs3 Crosslinking 
Sort   by:  
 Page size