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ProteomicsFLAG-PHF3 interactome in HEK293 cells
FLAG-hPHF3 was transiently expressed in human HEK293 cells, followed by anti-FLAG immunoprecipitation, on-bead digest and NanoLC-MS analysis.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026292 | Pride
Human Anti-flag immunoprecipitation Nanolc-ms analysis On-bead digest 
UnknownSISCAPA peptide enrichment on magnetic beads using an in-line bead trap device.
Not available
S-EPMC2689780 | biostudies-literature
ProteomicsPseudomonas putida SQ1 pull-down
Pseduomonas putida SQ1 cell lysates grown on sulfoquinovose (SQ) were labelled with activity-based probe 3 and labelled proteins pulled-down using streptavidin beads. An on-bead digest was performed and the resulting peptides analysed by LCMS/MS.
ORGANISM(S): Pseudomonas putida 
2024-07-10 | PXD047091 | Pride
Activity-based probe  sulfoquinovosidase  pseudomonas putida sq1 
MetabolomicsMicrobial liberation of N-methylserotonin from orange fiber in gnotobiotic mice and humans
Plant fibers in byproduct streams produced by non-harsh food processing methods represent biorepositories of diverse, naturally occurring, and physiologically active biomolecules. To demonstrate one approach for their characterization, mass spectrometry of intestinal contents from gnotobiotic mice, ...
2022-07-28 | MTBLS2331 | MetaboLights
UnknownWhat is the best technic to dislodge Staphylococcus epidermidis biofilm on medical implants?
Not available
S-EPMC9356421 | biostudies-literature
ProteomicsTargeting Ras-, Rho-, and Rab-Family GTPases via a Conserved Cryptic Pocket
The family of Ras-like GTPases consists of over 150 different members, regulated by an even larger number of guanine exchange factors (GEFs) and GTPase-activating proteins (GAPs) which comprise cellular switch networks that govern cell motility, growth, polarity, protein trafficking, and gene expres...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD054414 | Pride
Gtpase 
ProteomicsChemical acetylation of ligands and two-step digestion protocol for reducing co-digestion in affinity purification-mass spectrometry
We present an effective, fast and user-friendly method to reduce co-digestion of bead-bound ligands, such as antibodies or streptavidin, in affinity purification-mass spectrometry experiments. A short pre-incubation of beads with Sulfo-NHS-Acetate leads to chemical acetylation of lysine residues, ma...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2023-10-23 | PXD043709 | Pride
Chemical acetylation Immunopurification Streptavidin Ligand Affinity purification Sulfo-nhs-acetate Nanobody Gfp 
UnknownPhagocytosis of microparticles increases responsiveness of macrophage-like cell lines U937 and THP-1 to bacterial lipopolysaccharide and lipopeptide.
Not available
S-EPMC7990916 | biostudies-literature
ProteomicsCell-type specific tagging of glycoproteins in murine-human co-culture secretome glycoproteomics
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected ce...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-09-10 | PXD035445 | Pride
Mcf7 Human-murine 4t1 Secretome Co-culture  glycoproteomics 
ProteomicsCell-type specific tagging of glycoproteins in murine-human co-culture samples glycoproteomics lysates
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected ce...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-09-10 | PXD035449 | Pride
Mcf7 Human-murine Lysate 4t1 Co-culture  glycoproteomics 
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